Küçük alt ünite ribozomal RNA gen analizine dayanarak Türkiye’nin Karadeniz kıyılarından Merlangius merlangus euxinus’u (Linnaeus, 1758) enfekte eden Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvasının moleküler karakterizasyonu

Türkiye sularında Hysterothylacium cinsinin taksonomisi eksik ve belirsizdir. Bu araştırmada Türkiye’nin Karadeniz kıyılarında avlanan Merlangius merlangus euxinus’da H. aduncum larvaları morfolojik olarak teşhis edildi. H. aduncum’un nükleer ribozomal ITS gen bölgesi (ITS-1, 5.8S subunit, ITS-2) ile küçük alt ünite mitokondriyal ribozomal RNA (rrnS) gen bölgesi çoğaltıldı ve sekanslandı. BLAST analizleri H. aduncum’un mevcut ITS dizilerilerinin daha önce Türkiye Karadeniz’den kayıt edilen H. aduncum’un referans dizisiyle (erişim numarası JX413596) aynı olduğunu gösterdi. Türkiye Karadeniz’den H. aduncum türünün rrnS geni (MK886768), H. aduncum'un Akdeniz (MF000685-MF000691) ile Çin sularındaki (MF140344) izolatları ile %97.94-99.56 arasında benzerlik gösterdi. Bununla birlikte, Türkiye Karadeniz’den elde edilen H. aduncum’un rrnS dizisi ile H. aduncum'un Akdeniz (MF000685-MF000691) ile Çin sularındaki (MF140344) diğer izolatlarının ikili karşılaştırmasında %0,2 ile %1,7 arasında farklılık gözlendi. Bu çalışma ile ilk kez Karadeniz'den H. aduncum’un mitokondriyal rrnS geni moleküler olarak karakterize edildi.

Anahtar Kelimeler:

Hysterothylacium aduncum, Moleküler Karakterizasyon, Mitokondriyal rrnS geni, Karadeniz, Türkiye

Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascarididae) larvae infecting Merlangius merlangus euxinus (Linnaeus, 1758) from the Turkish Black Sea coast based on mitochondrial small subunit ribosomal RNA gene analysis

The taxonomy of Hysterothylacium genus remains incomplete and unclear in Turkish waters. In the present study, H. adumcum larvae were morphologically identified from Merlangius merlangus euxinus in the Black Sea, Turkey. The nuclear ribosomal ITS regions (ITS-1, 5.8S subunit, ITS-2) and the small subunit of the mitochondrial ribosomal RNA (rrnS) gene of H. aduncum were amplified and sequenced. The BLAST analysis indicated that present ITS sequences of H. aduncum were identical to that of the reference sequence of H. aduncum (accession no JX413596) recorded previously from the Black Sea, Turkey. The present rrnS gene of H. aduncum from the Black Sea, Turkey (MK886768) showed 97.94 to 99.56% identity with isolates of H. aduncum from the Mediterranean Sea (MF000685-MF000691) and the Chinese waters (MF140344). Moreover, pairwise comparison between the rrnS sequences of the H. aduncum from the Black Sea, Turkey (MK886768) and others H. aduncum isolates from the Mediterranean Sea (MF000685-MF000691), the Chinese waters (MF140344) showed differences ranged from 0.2 and 1.7%. With the present study, H. aduncum from the Black Sea was characterized for the first time by sequencing of the mitochondrial rrnS gene.

Keywords:

Hysterothylacium aduncum, Molecular characterization, mitochondrial rrnS gene, Black Sea, Turkey,

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