Koçlarda Aglutinasyon ve Agar- Jel Diffuzyon Yöntemiyle Brucella Ovis Antikorlarının Saptanması Üzerinde Araştırmalar

Bu araştırma.da kullanılan 205 koç serumundan 185'i Et ve Balık Kurumu'na, Konya Cihanbeyli kazasından kesime gelen hayvanlardan, 15'i Enstitümüzde deneme hayvanı olarak kullanılan koçlardan ve 5'i de Adana Mercimek Harasından Lôboratuvarımıza muayene için gelen koç serumlarından sağlanmıştır. Bu serumlar aynı zamanda serum aglutinasyon ve agar jel diffuzyon test yöntemiyle Bruceila ovis'e karşı yoklanmış olup serum aglutinasyon testinde 166 serum 1/10-1/640 ara.sında değişen aglutinin titreleri göstermiştir. Bu testte 43 serum pozitif, 6 şüpheli ve 156 serum negatif reaksiyon verirken, Agar jel diffuzyon testinde 50 serum pozitif, 155 serum da negatif sonuç vermiştir. Aglutinasyon testinde şüpheli 5 serum negatif, 3 serum agar jel diffuzyon testinde pozitif reaksiyon vermiştir. Yine agar jel'de negatif olan bir serum aglutinasyonla pozitif sonuç vermiştir. 42 serum iki testte de parelel çalışmış olup uyum yüzde % 84 dür. Buna. göre agar jel diffuzyon testinin daha kesin sonuç vermesi, bu testin duyarlı, basit ve ekonomik oluşuyla serolajik kanıda önemli bir yeri olduğu kanısına varılmıştır.

Anahtar Kelimeler:

Koç, Brucella Ovis, Brucella Ovis Antikor Saptanması, Mübeccel YUMUŞAK, 1984

Studies on aglutination and agar- gel diffusion test for the detection of antibodies against Brucella ovis in rams.

This study ıwas carried out using 205 blood serum of ram collected mainly from Konya, Ankara and Adana proices of the country. All serums ware examined both by serum aglutination and agar-gel diffusion tests comparisonly Br. ovis By serum aglutination tests 166 serums showed 1/10-1/640 agglutinin titres among them 43 serum !were positives, 156 negatif and 6 suspicion reactions were obtained. By agar gel diffusion test, 50 serum gave positive reactions and 155 ıwere negatives from 5 serums of suspicion and from 3 negatives reactions by agglutination tests were positives by agar gel diffussion tests on the otherland one serum sample showed negative reactions by agar gel diffuion test ıwas positive by agglutination test 42 (84) of the pasitives serums ıwere corresponded by both tıwo tests used. it seemed to be a.gar gel diffusion tests more sensitive simplier and economic, a.nd more valid than aglutinin tests for Bruceila ovis.

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1 - Altan, G.G. Jones L.M .• Pietz, D.E. (1975) : Lôboratory Tecmigues in Brucellosis. WHO Monograph Series. Second Edition, Geneva.
2 - B!berstein, E.L. and Mc Gowan, B. (1958) : Epididmitis ·in rams studies ve Lôboratory diagnosis. Cornell. Vet., 48 : 31.
3 - Biberstein, E.L. Mc Gowan, B., Robinson, E.A. and Harold. D.R. (1962) Epididymitis in rams. Studies on immunity. Cornell. Vet., 52: 214.
4 - Buchanan, R.E., Gibbans, N.E. (1975) : Bergeys Manuel of Determinative Bacteriology, Eighhh ed!dion. The Willams, Wilkins Company/ Baltimore.
5 - Buddle, M.B. and Boyes B.W. (1953) : A bruceila mutant oausing genital disease of sheep in Newzealand. Aust. Vet. J .• 29-145.
6- Clapp, K.H., Symons, E.A. and Doolette, J.B. (1955) : The application of a complement-Fixastion Test to. The Diagnosis of ovine Bruceilasis Aust. Vet. J., 31 : 29.
7 - Corbel, M.J., Gill, K.P.W. and Redwood, D.W. (1979) : Diagnostic procdure for non-Smooth Bruceila strains. Research sertion Disase of breeding department Central Veterinary Laboratory. New Haw 'Weybridge Surrey .
8 - Cox, C., J., Gorried, C.J.R., Naırn, R.C. and Word, H.A. (1977) : A Conparasıon of methods for the serological diagnosis of Bruceila ovis infection. Br. Vet J., 133: 442.
9 - Doğuer, M. (1961 l : Türkiye'de izole edilen Bruceila suşlarının idantifikasyonlarını . Etlik Vet. Bakt. Enst. Derg. 1 : (3) : 155.
10 - Hall, W.T.K. (1955) Epid!dymitis of rams. Studies 10 skin sensitivity and pathology, Aust. Vet. J .. 31 : 7.
11 - Hughes, K.L. and, Cloxton, P.D (1968) : Bruceila ovis infection. An evaluation of microbiological, serological An Clinical methods of diagnosis in the ranı . Aust. Vet. J., 54 : 41.
12 - Kenedy, P.C., Frazıer, L.M. and Mc Gowan, B. (1956) : Epididynıitis in roms : Patohology and Bacteriology, Cornell. Vet., 46 : 303.
13 - Keogh, J ,, Doolette, J.B. and Clapp. K.H. (1958) : The ep i dinıiology of ovinel Bruceilasis in soufh, Austiralia. Aust. Vet. J., 34 : 412
14· - Lawrence, W.E .. (1961) : ov!ne. Brucellosis. A rewiew of the disease in sheep nıanifested by ep i jidynıitis and abortion. Brit. Vet. J., 117 : 435.
15- Lows, L., Sinınıons , G.C., and Ludford, G.G .. (1972 ) : Experimental Bruceila ovis infeelian in Ranıs , Aust. Vet. J., 48 : 313.
16 - Maslarski, N. (1973) : Antibodies in the senıinal plasnıa or roms infected with Bruceila ovis. Veterinama Shirka .. 70 : 13. (Vet. Bull. 3850 abstraktan alınmıştır . )
17 - Matthews, T.R. and Trueblood, M.S. (1966 Detection of precipitins against Bruceila ovis. Cornell Vet., 57 : 410.
18 - Mc Gowan, B. and Shultz, G. (1956) : Ep i didynıitJs of ronıs. Clinical descripiian and field aspectes. Cornell. Vet .. 41 : 277.
19 - Meinershagen, W.A., Frank, F.W. and Waldhalnı, D.G. ( 1974) ovis as o cause of abertion inewes Aıner . J. Vet. Res .. 35. (5) Bruceila 723.
20 - Miller, S.J. and Moule, G.R. (1954 ) : Clinical observations on the reproductive organs of Merino Ranıs in Postoral Oueensland. Aust. Vet. J., 30 : 353.
21 - Myers, D.M. (1973) : Field Evaluation of the gel diffusion Test for rhe Diagnosis of Ranı Epidid y nıitis cauded by Bruceila ovis. Appl. Microbiol., 26, (6 ) : 855.
22 - Myers, D.M., Jones, L.M., Diaz, V. (1972) : Studies of antigens for conıpl e nıent Fixation and gel diffusion Test in the Diagnosis of in infections causej by Bruceila ovis and other Brucella. Appl. Microbiol .. 23 : 894.
23 - Myers, D.M. and Siniuk, A. (1970) : Preliminary Report on the Development of o Diffusion in gel Metbod for the Diagnosis of Ram Epid idymitis. Appl. Microbiol. 19 : 335,
24 - Muncz, F .• Körmendy, B. and Mascy. M. (1979) : Comparison of agar gel propıcıtation and coınplement fixation tlst for t-he detection of Bruceila ovis infection. Acta microbiol. Acad. Shi. hung .. 26 : 11.
25 - Osborne. H.G. ( 1955) : Epididymitis of Ram s. Au st. Vet. J., 31 : 11
26 - Renoux, G. and Mahaffey, L.W. (1955) : Sur Lexistence Probablede nauveau antigens des Brfuceila avec unpronuveau schema propose. Pour representer la repartition de antigens-Ann. inst. Pasteur. 8 : 528.
27 - Ris. R. (1963) : Diagnosis of Bruceila ovis !nfection in rams. Buil. World Health. organization. 5.2.3. WHO/ Brucellose/1228.
28 - Ris, R. (1968) : Bruceila ovis'in rams. V.PH/ WP/68 : 9.
29 - Sarısayın. F. (1969) : Koyun bruceilasis alanındaki son gelişmeler Semineri. T.B.T.A. Veterinerlik ve Hayvancılık Araştırma Grubu yayın No : 2.
30 - Shimuzu, T. and Shibata , S. (1962) : A techn!que of agglutination test whit R-type brucellas as antigen . Not. inst. Anim . Hltk. Ouart. 2 ( 1) : 15
31 - Simmons. G.C. and Hall. W.T.K. ( 1953) : Epididymitis of Rams Preliminary Preliminary Stud ies on the occurence and Pathogenicity or o rBrucella~like organizm. Aust. Vet. J. 29 : 33.
32 - Snowden, W.A. ( 1958) : Opening of Discussi on Au st. Vet. J . 34 : 417
33 - Stableforth. A.W. (1959) : infections diesases of animals Diseases Due to bacteria Vol. 1. Butter Worths Scientific Publications London.
34 - Van Heerden, K.M. (1963) : The immunization of Sheep against Br. ovis infection College og agricultre, Middelburg, South Africa . WHO/Bruc/ 239.
35 - Watt, D.A. (1972) : Testucular abnormalities and Spermatogenesis of avine and other Species. Vet. Bul!., 42, (4) : 181 .
36 - Wilson, G.S. and Miles. A.A. (1966) : Topley and Wilson's Principles of ·bacteriology and immunity. Vii. 1. Fifth edi tion, London Edvard Arnold Puplishers.
37 - Woernle, H. (1972) : Agar-gel diffusion Technique Staatl. Tierarztliches Untersuchungsamt Stutgart. Stutgart. 1. Azlenbergstrasse 16.