Veli GÜLYAZ, Fahriye SARAÇ, Mustafa HASÖKSÜZ, Serdar UZAR

Contagious Ektima (CE) aşısının bağışıklık ve zararsızlık çalışmalarında tavşanların kullanılması

Contagious Ektima (CE) hastalığı, genç koyun ve oğlaklarda lokalize kutanöz enfeksiyonlara sebebiyet veren, dünyada yaygın olarak bulunan Parapoxvirusların neden olduğu zoonotik viral bir enfeksiyondur. Bu çalışmanın amacı, CE aşısının üretilmesinden sonra bağışıklık ve güvenlik çalışmalarında kuzu ve oğlakların yerine tavşan kullanmaktı. Çalışmada kullanılan kuzulardan izole edilmiş patojen Pendik CE suşu (E(P)CK 4 ) ve attenüe olan (E(P)CK 22 ) aşı suşunun titrelerinin sırasıyla DKID 50 10 6.50 ve 10 7.00 /ml olduğu belirlendi. Tavşanlarda CE virusunun (E(P)CK 4 ) patojenitesini belirleme çalışmasında, CE virusuna özgü hiperemi, veziküller ve püstüller gözlemlenmedi, vücut sıcaklıklarında artış olmadı ve bel bölgesinde bulunan yara kabuklarında CE virusu PCR ile tespit edilmedi. Patojen CE virusu uygulanan ve CE aşı virusu ile aşılanan tavşanların kan serumlarında, CE virusuna karşı antikor tespit edilmedi. CE aşısı (E (P) CK 22 ) ile aşılanmış kuzu ve oğlaklarda yapılan bağışıklık çalışmasında, skarifikasyon yapılan deri bölgesinde veziküllerin, püstüllerin ve kabukların 3. günde ortaya çıktığı ve lezyonların 15. günde iyileştiği tespit edildi. CE aşı virusunun kuzu ve oğlakları patojen CE (E (P) CK 4 ) virusuna karşı koruduğu saptandı.

Anahtar Kelimeler:

Aşı, bağışıklık, contagious ektima, kuzu, oğlak, tavşan

The Use of Rabbits in Studies of Immunity and Safety of Contagious Ecthyma (CE) Vaccine

Contagious ecthyma (CE) is a zoonotic viral infection and common localized cutaneous infection of young sheep and goats caused by a Parapoxvirus with worldwide distribution. The aim of this study was to use rabbits instead of lambs and kids in immunity and safety studies after production of the CE vaccine. The titres of Pendik CE pathogen strain isolated lamb (E(P)CK4) and attenuated vaccine strain originated lamb (E(P)CK22) used in the study were TCID50 106.50 and 107.00/ml, respectively. In the study to determine the pathogenicity of CE virus (E(P)CK4) in rabbits, it was found that CE virus-specific hyperemia, vesicles and pustules were not observed and did not contain CE virus by PCR test in the scabs in the scarred back-waist regions, and body temperature did not increase in rabbits. In the blood sera of rabbits treated with pathogen CE virus and vaccinated with CE vaccine virus, no antibodies were detected against CE virus. It was found that the vesicles, pustules and scabs appeared on day 3 and the lesions healed on the 15th day. In the immunity study in lambs and kids vaccinated with CE vaccine (E(P)CK22), it was found that CE vaccine virus protects lambs and kids against pathogen CE (E(P)CK4) strain.

Keywords:

Contagious ecthyma, immunity, kid, lamb, rabbit, vaccine,

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