Bir Koyun Abort Fötusunun Border Disease Virusu ve Brucella melitensis ile Koenfeksiyonu

Bu çalışmada, bir koyun abort vakasında border disease virusu (BDV) ve Brucella melitensis enfeksiyonlarının potansiyel rolleri araştırılmıştır. İki bin on yedi yılında Türkiye'nin İç Anadolu Bölgesinde yer alan Konya İlindeki bir koyun işletmesinden, bir abort koyun fötusuna ait iç organ örnekleri ve fötusun annesinden EDTA'lı tam kan örneği elde edilmiştir. Brucella spp. varlığı bakteriyel izolasyon, BDV ise real time RT-PCR yöntemi ile araştırılmıştır. BDV'unun genetik karakterizasyonu, 5'- translate olmayan bölge sonunun (UTR) sekans analizi ile gerçekleştirilmiştir. Abort koyun fötusundan izole edilen Brucella suşu biyokimyasal karakteri ve monospesifik A ve M serumları ile aglütinasyonuna bağlı olarak Brucella melitensis olarak identifiye edilmiştir. Koyun abort fötusunda ve EDTA'lı kan örneğinde BDV tespit edilmiştir. Filogenetik analiz sonucu, bu çalışmada izole edilen BDV saha suşunun BDV-7 genotipinde olduğu belirlenmiştir. Bizim bildiğimiz kadarıyla, bu çalışma koyun fötusunun BDV ve Brucella melitensis ile birlikte enfeksiyonu hakkındaki ilk rapordur

Co-infection with Border Disease Virus and Brucella melitensis in an Aborted Sheep Foetus

In this study, we investigated the potential roles of border disease virus (BDV) and Brucella melitensis infections in a case of sheep abortion. Internal organ specimens from aborted sheep foetus and EDTA whole blood sample from mother of the foetus were collected from a sheep flock in the Konya Province in the Central Anatolia region of Turkey in 2017. The presence of Brucella spp. directly assessed by bacterial isolation and detection of BDV was carried by real time RT-PCR. Genetic characterization of the BDV field isolate was conducted by sequencing the 5’- end untranslated region (UTR) region of BDV. Brucella strain was isolated from the samples of aborted sheep foetus, and it was identified as Brucella melitensis by biochemical characteristics, agglutination with monospecific A and M sera. BDV RNA was detected in EDTA whole blood sample and aborted sheep foetus. Phylogenetic analysis in 5’-UTR region allocated the field isolate of BDV obtained in this study into BDV-7 genotype. To the best of our knowledge, this is the first report on the dual infection of aborted sheep foetus with BDV and Brucella melitensis

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