Lemna minor, Lemna gibba and Spirodela polyrrhiza (L.)'da Akümülasyon, Antioksidan Enzim Aktivitesi ve MDA içeriğine Bakırın Etkileri

Lemna minor, Lemna gibba ve Spirodela polyrrhiza L. bitkilerinde akümülasyon, antioksidan enzim aktivitesi, MDA miktarı, klorofil ve karoteniod içeriği üzerine farklı konsantrasyonlardaki bakırın etkileri incelenmiştir. Su mercimeklerine dört gün boyunca farklı konsatrasyonlarda bakır uygulanmıştır. Elde edilen sonuçlara göre; bakır iyonları L. minor, L. gibba ve S. polyrrhiza oldukça fazla miktarda biriktiği belirlenmiştir. MDA içeriği ve prolin miktarı su mercimeklerinde artan dış bakır konsantrasyonunun artışıyla birlikte yükselmektedir. Fakat bununla birlikte fotosentez pigment derecesi artan bakır konsantrasyonuyla düşüş göstermiştir. Bitkilerin 25-50 µM Cu uygulaması diğer uygulamalarla karşılaştırıldığında süperoksit dismutaz ve katalaz enzim aktivitesi önemli bir oranda artış göstermiştir. Bu sonuçlar bize L. minor, L. gibba ve S. polyrrhiza bitkilerinin 25-50 µM Cu uygulamasını tolere edebileceğini ifade etmektedir.

Effects of Copper on Accumulation, Antioxidant Activity and MDA Content in Lemna minor, Lemna gibba and Spirodela polyrrhiza (L.)

The effects of different concentrations of copper on the accumulation, antioxidant enzyme activity, malondialdehyde (MDA), chlorophyll and carotenoid content of Lemna minor, Lemna gibba and Spirodela polyrrhiza L. were investigated. Duckweeds were treated with several concentrations of Cu (0, 10, 25, 50, and 100 µM Cu) for 4 days. According to results, Cu ions were accumulated mainly in L. minor, L. gibba and S. polyrrhiza. The MDA content and proline amount increased with increasing external Cu concentration in duckweeds. However the level of photosynthetic pigments decreased with increasing Cu concentration. Superoxide dismutase and catalase antioxidative enzyme activity increased significantly in plants exposed to 25-50 µM Cu compared to other treatments. These results show that L. minor, L. gibba and S. polyrrhiza can tolerate up to 25 µM Cu treatment.

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