Hatice Kübra BAŞALOĞLU, HULKİ BAŞALOĞLU, MEHMET TURGUT, AYŞEGÜL UYSAL, Mine Ertem YURTSEVEN

Ultrastructural effects of nicotine administration on sympathetic nervous system of adrenal gland medula in fats: A qualitative study by electron microscopy

Subkutan nikotin enjeksiyonu uygulanan Wistar cinsi sıçanlarda surrénal medulla sempatik sinir sistemi morfolojisi tetkik edilmiştir. Hayvanlara 7 hafta süreyle düşük dozda (2 mg/kg/gün) ve yüksek dozda (4 mg/kg/gün) nikotin subkutan olarak uygulanmıştır. Surrénal bezlerin medullasındaki morfolojik değişiklikler elektron mikroskop kullanılarak ayrıntılı bir şekilde değerlendirilmiştir. Ultrastrüktürel olarak, epinefrin (E) ve norepinefrin (NE) içeren hücrelere ait sinüzoidlerin ve endotel hücrelerinin normal görünümde olduğu saptanmıştır. Hem düşük ve hem de yüksek doz nikotin uygulanan tedavi gruplarında, E ve NE salgılayan hücreler içinde yoğun granülasyon görülmüştür. Ayrıca, surrénal medulla sinüzoidlerindeki genişlemeye ilave olarak, özellikle NE salgılayan hücrelerdeki sekresyon artışı nedeniyle ortaya çıkan aşırı bir vakuolizasyon ve nukleus içi depolanmalar dikkati çekmiştir. Yüksek doz nikotin uygulanan hayvanlarda sinüzoidlerin çevrelerinde yer alan endotel hücrelerinin duvarlarında bir hipertrofı mevcudiyeti saptanmıştır. Bu bulgularla, sıçanlarda nikotinin surrénal medulla sempatik sinir sistemi üzerinde doza-bağımlı olan ve antihipertansif bir etki gösterdiğini düşündüren belirgin morfolojik değişikliklerin geliştiği sonucuna varılmıştır.

Anahtar Kelimeler:

Nikotin, Böbrek üstü bezi medullası, Sempatik sinir sistemi, Mikroskopi, elektron, Sıçan, Wistar, Modeller, hayvan, Modeller, teorik

Sıçanlarda nikotin uygulamasının sürrenal bez medullasının sempatik sinir sistemi üzerine etkileri: Elektron mikroskopla gerçekleştirilen kalitatif bir çalışma

Morphology of sympathetic nervous system of adrenal medulla was observed in Wistar rats injected with subcutaneous nicotine. They were injected subcutaneously with low dose (2 mg/kg/day) or high dose (4 mg/kg/day) nicotine for seven weeks. Morphological changes in the medulla of adrenal glands were investigated in detail using electron microscopy. Ultrastructurally, we found that the appearance of sinusoids and endothelial cells containing epinephrine (E) and norepineprine (NE) granules were normal. In both low and high dose nicotine treatment groups, the granules within cells secreting E and NE were dense. Furthermore, in addition to an enlargment in the sinusoids of the adrenal medulla, depositions in the nuclei and an excessive vacuolisation due to increased secretion especially in NE secreting cells were noted. There was also hypertrophy of the walls of endothelial cells around sinusoids in animals treated with high-dose nicotine. We conclude that nicotine has a dose-dependent effect causing obvious morphological changes on the sympathetic nervous system of adrenal medulla in rats, suggesting its hypertensive effect.

Keywords:

Nicotine, Adrenal Medulla, Sympathetic Nervous System, Microscopy, Electron, Rats, Wistar, Models, Animal, Models, Theoretical,

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