Metisilin Dirençli Staphylococcus aureus Suşlarına karşı Karvakrol ve Oksasilinin Kombinasyon Aktivitesinin İn Vitro Değerlendirilmesi

Amaç: Metisilin dirençli Staphylococcus aureus (MRSA) antibiyotiklere en dirençli bakterilerden biridir. Birçok antibiyotiğin bu bakterilerin neden olduğu infeksiyonları tedavi etmede yetersiz kaldığı bilinmektedir. Bitki türevli antibakteriyeller yeni terapötiklerin kaynağı olarak dikkat çekmektedirler. Karvakrol, çeşitli uçucu yağlarda bulunan monoterpenik fenol bileşiğidir ve birçok patojene karşı antimikrobiyal aktivite göstermektedir. Bu çalışmada on klinik MRSA suşuna karşı karvakrol ve oksasilinin kombinasyon aktivitesi değerlendirilmiştir. Gereç ve Yöntemler: MRSA suşlarına karşı karvakrol ve oksasilinin Minimum İnhibitör Konsantrasyonlarını (MİK) saptamak için broth mikrodilüsyon metodu uygulanmıştır. Karvakrol ve oksasilinin kombinasyon aktivitesi checkerboard sinerji testi ile belirlenmiştir. Her iki antimikrobiyalin ve kombinasyonlarının MRSA-6 suşunda membran hasarına neden olup olmadığı ise membran boyunca hücre dışına sızan nükleik asit miktarının UV spektrofotometre ile ölçülmesiyle saptanmıştır. Bulgular: Karvakrol 64-256 µg/ml aralığındaki MİK değerleri ile tüm MRSA suşlarına karşı antibakteriyel aktivite göstermiştir. Karvakrol ve oksasilinin yedi suşa karşı sinerjistik etki gösterdiği saptanmıştır (FICI≤0,5). Karvakrol, MRSA-6 suşunda membran hasarına neden olmuştur. Oksasilin ile kombinasyonu sonucunda, MRSA-6 suşunun membran hasarındaki artış istatistiksel olarak anlamlı bulunmuştur (p

In Vitro Evaluation of the Combination Activity of Carvacrol and Oxacillin against Methicillin-Resistant Staphylococcus aureus Strains

Aim: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most resistant bacteria to antibiotics. Manyantibiotics are known to be insufficient to treat infections caused by these bacteria. Plant-derived antibacterials havedrawn more attention as a source of new therapeutics. Carvacrol is a monoterpenic phenol compound found in variousessential oils and shows antimicrobial activity against many pathogens. In this study, the combination activity ofcarvacrol and oxacillin against ten MRSA clinical strains was evaluated.Material and Methods: To determine Minimum Inhibitory Concentrations (MIC) of carvacrol and oxacillin against tenMRSA clinical strains, broth microdilution method was performed. The combination activity of carvacrol and oxacillinwas determined with checkerboard synergy test. Whether both antimicrobials and their combinations caused membranedamage in MRSA-6 strain was detected by measuring the amount of nucleic acid leaking out of the cell acrossmembrane with UV spectrophotometer.Results: Carvacrol showed antibacterial activity against all MRSA strains with MIC values in the range of 64-256µg/ml. The synergistic effect (FICI≤0.5) between carvacrol and oxacillin was determined against seven strains.Carvacrol caused a membrane damage on MRSA-6 strain. As a result of combination with oxacillin, the increase inthe membrane damage of MRSA-6 strain was found to be statistically significant (p

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