Mustafa YILDIRIM, İrfan SENCAN, Sükrü ÖKSÜZ

Hastane Personelinde Fekal Vankomisin Dirençli Enterokok Kolonizasyonunun Arastirilmasi

Bu çalismada, Düzce Tip Fakültesinde hastane personelinde fekal vankomisin dirençli enterokok (VRE) kolonizasyonu arastirilmistir. Diski kültürlerinden enterokok izole edilen 62 hastane personeli ve 37 gönüllü bu çalismaya alindi. Diski örneklerinden enterokok kökenlerinin identifikasyonunda klasik mikrobiyolojik yöntemler ve API 20 Strep testi kullanildi. Antimikrobiyal duyarlilik testleri disk difüzyon yöntemiyle yapildi. Betalaktamaz aktivitesi nitrosefin diskleriyle çalisildi. Izole edilen toplam enterokok kökenlerinin türlere göre dagilimi: 56 (%56.6) E.faecium, 24 (%24.2) E.gallinarum, 13 (%13.1) E.faecalis, 5 (%5.1) E.durans, 1 (%1.0) E.casseliflavus olarak belirlenmistir. Hastane personelinden izole edilen tüm enterokok kökenleri arasinda ampisilin direnci %12.9, Yüksek düzey streptomisin (YDS) direnci %19.4 ve Yüksek düzey gentamisin (YDG) direnci %1.6 olarak saptanmistir. YDS direnci hastane personelinde kontrol grubuna göre anlamli sekilde yüksek bulundu (x2:8.149, p=0.002). Hiçbir enterokok susunda vankomisin direnci ve betalaktamaz aktivitesi saptanma mistir. Sonuç olarak, hastane personelinde fekal VRE tasiyiciligi saptanmadi. Ancak periyodik tarama çalismalarinin sürdürülmesinin dirençli kökenlerin erken saptanmasi açisindan gerekli olduguna inaniyoruz

Anahtar Kelimeler:

Enterokok, hastane personeli, diski kolonizasyonu

The Investigation Of Fecal Vancomycin Resistant Enterococcus Colonization In Hospital Personnel

In this study, the prevalence of fecal vancomycin resistant enterococcus (VRE) colonization was investigated in hospital personnel in Duzce Medical Faculty. Sixty two hospital personnel and 37 volunteer whom enterococcus species had isolated from stool samples were included in the study. The classical microbiological methods and API 20 Strep was used for the identification of enterococcus species from stool samples. Susceptibility was determined by the disk diffusion method. The beta lactamase activitywas tested with nitrocephin disk. According to our results 56 (56.6%) of the isolated enterococcus species was identified as E.faecium, 24 (24.2) % as E.gallinarum, 13 (13.1%) as E.faecalis, 5 (%5.1) as E.durans, and 1 (%1.0) E.casseliflavus. Resistance to ampicillin was found in 12.9% of samples. High-level resistance to gentamicin was found in 9.9% of samples. 19.8% of samples were found to be highly resistant to streptomycin. High-level resistance to streptomycin was higher in hospital personnel than control group (x2:8.149, p=0.002). No vancomycin resistance was noted. It was found that none of the enterococci had beta-lactamase enzyme activity. Our results demonstrated that there is no fecal carriage of VRE in hospital personnel. However we suggest that the screening tests should be done periodically to reveal resistant strains early

Keywords:

Enterococcus, hospital personnel, stool colonization,

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