Keklik (Alectoris chukar) Bağırsağında Ghrelin, Leptin ve Obestatin Dağılımı

Leptin, esas olarak yağ doku tarafından üretilen bir hormondur, ancak ghrelin ve obestatin temel olarak mideden izole edilmiştir. Bu hormonlar ayrıca gastrointestinal sistem, plasenta, iskelet kası ve meme epiteli gibi birçok dokudan da sentezlendiğinden bunların daha geniş bir rolü olduğu düşünülür. Bu yüzden, bu çalışmanın amacı, keklik bağırsağındaki leptin, ghr elin ve obestatin üreten hücrelerin dağılımını değerlendirmektir. 10 erişkin keklik bağırsağı (5 dişi ve 5 erkek), immünohistokimyasal yöntemle r kullanılarak ışık mikroskobu düzeyinde incelenmiştir. Ghrelin, leptin ve obestatin immünoreaktivitesi, ince ve kalın bağırsaktaki epitel, bazı stromal ve düz kas hücrelerinde gözlendi. Ayrıca, kan damarlarının endotel hücreleri, leptin için orta derecede immünoreaktivite gösterdi. Bununla birlikte, ghrelin için immün boyanma, ince ve kalın bağırsağın düz kas hücrel erinde belirgin bir şekilde tespit edildi. İlginç bir şekilde, obestatin için bazı immunpozitif hücreler sadece epitelde görüldü. Bu çalışma, ghrelin, leptin ve obestatinin bağırsakta farklı bir dağılım sergilediğini ortaya koydu. Bu bulgular, ghrelin, lep tin ve obestatinin gastrointestinal fonksiyonların düzenlenmesinde fizyolojik bir rol oynayabileceğini düşündürmektedir.

Distribution of Ghrelin, Leptin and Obestatin in the Intestine of Chukar Partridge (Alectoris chukar)

Leptin is a hormone that is mainly produced by the adipose tissue but ghrelin and obestatin have been isolated fundamentally from the stomach. These hormones are also synthesized in many other tissues, including gas trointestinal tract, placenta, skeletal muscle, and mammary epithelium, thus suggesting a wider role of these proteins. So the aim of this study was to evaluate the distribution of leptin, ghrelin and obestatin producing cells in the intestine of chukar pa rtridge. The intestines of 10 adult chukar partridge (5 male and 5 female) was examined on the level of light microscope by using immunohistochemical methods. Ghrelin, leptin and obestatin immunoreactivity were observed in the epithelial, some stromal and smooth muscle cells in the small and large intestine. Besides, endothelial cells of blood vessels showed moderate immunoreactivity for leptin. However, immunostaining for ghrelin was prominently detected in the smooth muscle cells of the small and large in testine. Interestingly, some immunopositive cells for obestatin was only seen in the epithelium. The present study revealed that ghrelin, leptin and obestatin exhibit a different distribution of the intestine. These findings suggest that ghrelin, leptin and obestatin may play a physiological role in the regulation of gastrointestinal functions.

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