Ünal YAVUZ, Füsun, Akın YİĞİN, NİHAT YUMUŞAK

Investigation of the Effects of Topical Centaurium Erythraea on Full-Thickness Skin Wounds Healing in Diabetic Rabbits

Centaurium erythraea, a species of flowering plant in the Gentianaceae family, is a plant commonly used in the wound treatment. This study aims to investigate the effect of Centaurium erythraea for enhancing healing process in full-thickness skin wounds in diabetic rabbits. A total of 28 young female New Zealand rabbits were used and animals were divided into four equal groups randomly. Diabetes mellitus was created by injection of alloxan monohydrate. After 15 days following diabetes induction, two full-thickness wounds, a diameter of 2.6 cm, were created equidistantly from the dorsal median line under general anesthesia. For wounds, on a daily basis, oily homogenized form of Centaurium erythraea was administered in Group 1 (n=7), while pomade form of the titrated extract of Centella asiatica in Group 2 (n=7), pure olive oil in Group 3 (n=7) and normal saline was applied in Group 4 (n=7). Wound edges were measured on the 4th, 8th, 12th, 16th, 20th and 24th days, furthermore, histopathological and genetic examinations were performed on tissue samples taken on the same days. Examining the wound diameters, healing rates in the Group 1 and Group 2 were found statistically significant compared to Group 3 and Group 4 (p

Diyabetik Tavşanların Tam Katlı Deri Yaralarının İyileşmesine Topikal Centaurium Erythraea’nın Etkisinin Araştırılması

Gentianaceae ailesine ait olan Centaurium erythraea yara tedavisinde halk arasında sık kullanılan bir bitkidir. Bu çalışmanın amacı Centaurium erythraea’nın diyabet oluşturulmuş tavşanların tam katlı deri yaralarında yara iyileşmesi üzerine etkisini araştırmaktır. Çalışmada 28 adet Yeni Zelanda tavşanı kullanıldı ve hayvanlar rastgele dört eşit gruba ayrıldı. Alloksan monohidrat enjeksiyonlarıyla diyabet oluşturuldu. Diyabet indüksiyonundan 15 gün sonra genel anestezi altında dorsal median hattan eşit uzaklıkta iki adet 2,6 cm çapında tam katlı deri yaraları oluşturuldu. Yaralara günlük olarak Grup 1 (n=7) Centaurium erythraea’nın yağlı homojenize formu, Grup 2 (n=7) Centella asiatica’nın titre edilmiş ekstraktının pomat formu, Grup 3 (n=7) saf zeytinyağı ve Grup 4 (n=7) serum fizyolojik uygulandı. Yara sınırları 4, 8, 12, 16, 20 ve 24. günlerde ölçülürken aynı günlerde alınan doku örneklerinde histopatolojik ve genetik incelemeler yapıldı. Çalışma sonunda yara çapları incelendiğinde Grup 1 ve Grup 2’de yara iyileşme hızı Grup 3 ve Grup 4’e oranla istatistiksel olarak önemli bulundu (p

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1. Hamza N, Berke B, Cheze C, et al. (2010). Prevention of Type 2 Diabetes Induced by High Fat Diet in the C57BL/6J Mouse by Two Medicinal Plants Used in Traditional Treatment of Diabetes in the East of Algeria. J Ethnopharmacol. 128(2): 513-518.

2. Perez-Nieves M, Ivanova JI, Hadjiyianni I, et al. (2017). Basal Insulin Initiation Use and Experience Among People with Type 2 Diabetes Mellitus with Different Patterns of Persistence: Results from a Multinational Survey. Curr Med Res Opin. 33(10): 1833-1842.

3. Yadav M, Sharma N, Garg A, et al. (2017). Herbal Drugs and Phytoconstituents Useful for the Management of Diabetes. Int J Green Pharm. 11(1): 21-29.

4. Chouhan D, Janani G, Chakraborty B, Nandi SK, Mandal BB. (2018). Functionalized PVA–Silk Blended Nanofibrous Mats Promote Diabetic Wound Healing via Regulation of Extracellular Matrix and Tissue Remodelling. J Tissue Eng Regen Med. 12(3): 1559-1570.

5. Yadollah-Damavandi S, Chavoshi-Nejad M, Jangholi E, et al. (2015). Topical Hypericum perforatum Improves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model. Evid Based Complement Altern Med. Article ID 245328: 1-4.

6. McCarthy ME, Brown TA, Bukowska J, et al. (2018). Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering. Curr Stem Cell Rep. 4(2): 127-137.

7. Pradhan L, Cai X, Wu S, et al. (2011). Gene Expression of ProInflammatory Cytokines and Neuropeptides in Diabetic Wound Healing. J Surg Res. 167(2): 336-342.

8. Luckett-Chastain LR, Gipson JR, Gillaspy AF, Gallucci RM. (2018). Transcriptional Profiling of Irritant Contact Dermatitis (ICD) in a Mouse Model Identifies Specific Patterns of Gene Expression and İmmune-Regulation. Toxicology. 410: 1-9.

9. Law JX, Chowdhury SR, Aminuddin BS, Ruszymah BHI. (2017). Role of Plasma-Derived Fibrin on Keratinocyte and Fibroblast Wound Healing. Cell Tissue Bank. 18(4): 585-595.

10. Shi HX, Lin C, Lin BB, et al. (2013). The Anti-Scar Effects of Basic Fibroblast Growth Factor on The Wound Repair in Vitro and in Vivo. PloS one, 8(4): e59966.

11. Xu C, Rosler E, Jiang J, et al. (2005). Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned Medium. Stem cells, 23(3): 315- 323.

12. Vatankhah N, Jahangiri Y, Landry GJ, Moneta GL, Azarbal AF. (2017). Effect of Systemic Insulin Treatment on Diabetic Wound Healing. Wound Repair Regen, 25(2): 288-291.

13. Arulselvana P, Abdul Ghofara HA, Karthivashana G, et al. (2014). Antidiabetic Therapeutics from Natural Source: A Systematic Review. Biomed Prev Nutr. (4): 607–617.

14. Arumugam G, Manjula P, Paari N. (2013). A Review: Anti Diabetic Medicinal Plants Used for Diabetes Mellitus. J Acute Dis. 2(3): 196-200.

15. Grover JK, Yadav S, Vats V. (2002). Medicinal Plants of India with Anti-Diabetic Potential, J Ethnopharmacol. 81 (1): 81-100.

16. Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2001). Antioxidant Activity of Centaurium erythraea Infusion Evidenced by Its Superoxide Radical Scavenging and Xanthine Oxidase Inhibitory Activity. J Agric Food Chem. 49: 3476−3479.

17. Tahraoui A, Israili ZH, Lyoussi B. (2010). Acute and Sub-Chronic Toxicity of a Lyophilised Aqueous Extract of Centaurium erythraea in Rodents. J Ethnopharmacol. 132(1):48-55.

18. Springefeld K. Centaurium erythraea-The medicinal plant of the year 2004. Pharm Ztg. 149: 30.

19. Šiler B, Mišić D. (2016). Biologically Active Compounds from the Genus Centaurium sl (Gentianaceae): Current Knowledge and Future Prospects in Medicine. In: Studies in Natural Products Chemistry. Vol. 49, pp. 363-397. Elsevier, USA.

20. Tuluce Y, Ozkol H, Koyuncu I, Ine H. (2011). Gastroprotective Effect of Small Centaury (Centaurium Erythraea L) on AspirinInduced Gastric Damage in Rats. Toxıcol Ind Health. 27(8): 760- 768.

21. Berkan T, Üstünes L, Lermioglu F, Özer A. (1991). Antiinflammatory, Analgesic, and Antipyretic Effects of an Aqueous Extract of Erythraea centaurium. Planta Med. 57: 34-37.

22. Moore N, Hamza N, Berke B, Umar A. (2017). News from Tartary: an Ethnopharmacological Approach to Drug and Therapeutic Discovery. Br J Clin Pharmacol. 83(1): 33-37.

23. Schouppe D, Brys R, Vallejo-Marin M, Jacquemyn H. (2017). Geographic Variation in Floral Traits and the Capacity of Autonomous Selfing Across Allopatric and Sympatric Populations of Two Closely Related Centaurium Species. Sci Rep. 7: 46410.

24. Kumarasamy Y, Nahar L, Cox PJ, Jaspars M, Sarker SD. (2003). Bioactivity of Secoiridoid Glycosides from Centaurium erythraea. Phytomedicine. 10(4): 344-347.

25. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2003). Hydroxyl Radical and Hypochlorous Acid Scavenging Activity of Small Centaury (Centaurium erythraea) Infusion. A Comparative Study with Green Tea (Camellia sinensis). Phytomedicine. 10: 517–522.

26. Kumarasamy Y, Nahar L, Sarker SD. (2003). Bioactivity of Gentiopicroside from the Aerial Parts of Centaurium erythraea. Fitoterapia. 74: 151–154.

27. Stefkov G, Miova B, Dinevska-Kjovkarovska S, et al. (2014). Chemical Characterization of Centaurium Erythrea L. and its Effects on Carbohydrate and Lipid Metabolism in Experimental Diabetes. J Ethnopharmacol. 152: 71-77.

28. Banjanac T, Dragićević M, Šiler B, et al. (2017). Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search for High-Resolution Taxonomic Classifiers. Phytochemistry. 140: 27-44.

29. Breen A, Mc Redmond G, Dockery P, O’Brien T, Pandit A. (2008). Assessment of Wound Healing in the Alloxan-Induced Diabetic Rabbit Ear Model. J Invest Surg 21(5): 261-9.

30. Han MC, Durmuş AS, Sağlıyan A, et al. (2017). Effects of Nigella sativa and Hypericum perforatum on Wound Healing. Türk J Vet Anım Sci. 41: 99-105.

31. Hassani FV, Naseri V, Razavi BM, Mehri S, Abnous K, Hosseinzadeh H. (2014). Antidepressant Effects of Crocin and its Effects on Transcript and Protein Levels of CREB, BDNF, and VGF in Rat Hippocampus. DARU. 22(1): 16.

32. Karaca ZM, Ozen H, Akgoz M. Cigremis Y. (2018). Effect of Caffeic Acid Phenethyl ester (CAPE) on Vascular Endothelial Growth Factor a (VEGF-A) Gene Expression in Gentamicin-Induced Acute Renal Nephrotoxicity. Medicine. 7(4): 805-9.

33. Słotwiński R, Sarnecka A, Dąbrowska A, et al. (2015). Innate Immunity Gene Expression Changes in Critically Ill Patients with Sepsis and Disease-Related Malnutrition. Cent Eur J Immunol. 40(3): 311.

34. Hamza N, Berke B, Cheze C, et al. (2011). Treatment of High Fat Diet Induced Type 2 Diabetes in C57BL/6J Mice by Two Medicinal Plants Used in Traditional Treatment of Diabetes in The East of Algeria. J Ethnopharmacol. 133(2): 931-933.

35. Gopinath D, Ahmeda MR, Gomathia K, Chitraa K, Sehgalb PK, Jayakumara R. (2004). Dermal Wound Healing Processes with Curcumin Incorporated Collagen Films. Biomaterials. 25: 1911– 1917.

36. Shukla A, Rasik AM, Dhawan BN. (1999). Asiaticoside-Induced Elevation of Antioxidant Levels in Healing Wounds. Phytother Res. 13: 50–54.

37. Maquart FX, Bellon G, Wegrowski Y, Barel JP. (1990). Stimulation of Collagen Synthesis in Fibroblast Culture by a Triterpene Extracted from Centella asiatica. Conn. Tissue Res. 24: 107-120.

38. Nabzdyk LP, Kuchibhotla S, Guthrie P, et al. (2013). Expression of Neuropeptides and Cytokines in a Rabbit Model of Diabetic Neuroischemic Wound Healing. J Vasc Surg. 58(3): 766-775.

39. Lin ZQ, Kondo T, Ishida Y, Takayasu T, Mukaida N. (2003). Essential Involvement of IL-6 in the Skin Wound-Healing Process as Evidenced by Delayed Wound Healing in IL-6-Deficient Mice. J Leukoc Biol. 73(6): 713-721.

40. Lan CCE, Wu, CS, Huang SM, Wu, IH, Chen GS. (2013). High-Glucose Environment Enhanced Oxidative Stress and Increased Interleukin-8 Secretion from Keratinocytes: New Insights into Impaired Diabetic Wound Healing. Diabetes. 62(7): 2530-2538.

41. El Euch SK, Cieśla Ł, Bouzouita N. (2014). Free Radical Scavenging Fingerprints of Selected Aromatic and Medicinal Tunisian Plants Assessed by Means of TLC-DPPH• Test and Image Processing. J AOAC Int. 97(5): 1291-1298.

42. Sefi M, Fetoui H, Lachkar N, et al. (2011). Centaurium erythrea (Gentianaceae) Leaf Extract Alleviates Streptozotocin-Induced Oxidative Stress and Β-Cell Damage in Rat Pancreas. J Ethnopharmacol. 135(2): 243-250.

43. Shetty S, Udupa S, Udupa L. (2008). Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum Sanctum Linn in Rats. Evid Based Complement Altern Med. 5(1): 95-101.

44. Cos P, Ying L, Calomme M, et al. (1998). Structure−Activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine Oxidase and Superoxide Scavengers. J Nat Prod. 61(1): 71- 76.