Mehmet ÖZBEK, Emel ERGÜN, Levent ERGÜN, Feyzullah BEYAZ, Füsun ERHAN, Banu KANDİL, Nuh YILDIRIM, Özge ÖZGENÇ

Fötal gelişim boyunca koyun ileumundaki Toll-like reseptör 2 ekspresyonu

Toll like reseptörler endojen veya mikrobiyal kompanentlerle aktive olan patern tanıyan reseptörlerdir. Bu reseptörler arasında, TLR2 intestinal inflamasyon, nöromüsküler fonksiyon, enterik sinir sistemi yapısını ve nörokimyasal kodlamayı düzenler. Bu çalışmada, prenatal dönemde koyun ileumunda TLR2 ekspresyonunu immunohistokimyasal yöntemle araştırmayı amaçladık. Prenatal dönemi 3 ayrı bölümde inceledik (60-100, 100-125, 125-150). İntestinal ve folikülle ilişkili epitelde moderate boyanma gözlenirken, bazı epitel hücrelerinde yoğun intrasitoplazmik immun reaksiyon gözledik. Ayrıca, foliküllerdeki hücrelerin büyük çoğunluğunda pozitif reaksiyon belirlendi. Tunika muskularis, lamina muskularis ve damar duvarlarındaki düz kaslarda da pozitif reaksiyon gözlendi. Ayrıca, submukozal ve myenterik pleksuslardaki gangliyon hücreleride pozitif boyanma gösterdi. Sonuç olarak, bu çalışma prenatal gelişimde koyun ileumunda TLR2 ekspresyon profilini tanımlayan ilk araştırmadır. İleumdaki TLR2 ontogenezisin doğum öncesi dönemde başladığı gösterilmiştir. Hemen hemen tüm intestinal epitel hücreleri ve enterik nöronlar TLR2 ekspresyonu gösterdi. Düz kaslarda TLR2 ekspresyonunun varlığı, düz kasların kasılma görevi dışında bağışıklık sistemi ile ilişkili olabileceğini göstermektedir.

Anahtar Kelimeler:

İleum, M hücresi, Peyer plakları, TLR2

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