BİR SELF-ETCH ADEZİVİN FARKLI SAKLANMA KOŞULLARININ DENTİNE BAĞLANMA DAYANIMI ÜZERİNE ETKİSİ

Amaç: Çalışmanın amacı farklı koşullarda bekletilen bir Self-Etch adezivin dentin makaslama bağlanma dayanımına etkisini değerlendirmektir. Gereç ve Yöntem: Çalışmada çekilmiş 40 adet sağ- lam insan molar dişi kullanıldı. Dişlerin dentin yüzeyi açığa çıkarıldı ve oklüzal yüzeyleri üstte kalacak şekilde akrilik rezin bloklara gömüldü. Ardından örnekler rast- gele 5 gruba bölündü. Self-Etch adeziv (Clearfil S3 Bond, Kuraray Dental, Okayama, Japonya) ve bir nanohibrit kompozit (Grandio; Voco, Cuxhaven, Almanya) 3mm çapında, 4mm yüksekliğinde bir kalıp yardımıyla dentin yüzeyine uygulandı. Gruplardan biri- ne adeziv sürülmesinin ardından kompozit rezin uygu- lanarak LED ışık cihazıyla (3M Espe, Seefeld, Almanya) polimerizasyon tamamlandı. 24 saat distile suda bek- letilen örneklerin universal test cihazı (Instron Corp. Canton, MA) ile makaslama bağlanma dayanımı ölçül- dü. Ardından adezivlerden biri buzdolabında, diğeri ise oda sıcaklığında bekletildi. 1 ay ve 6 ay sonunda her adeziv birer gruba uygulanarak makaslama bağlanma dayanımı yeniden ölçüldü. Veriler kaydedilerek SPSS 20 yazılımıyla tek yönlü Varyans analizi yapıldı (α=0.05). Bulgular: En yüksek bağlanma dayanımı 24 saat sonra hazırlanan örneklerde görüldü (18,35 MPa). 1 ay sonrasında, test sonuçlarına göre buzdolabı sıcaklığında bekletilen adeziv ile hazırlanan örneklerle oda sıcaklığında bekletilen adeziv ile hazırlanan örnekler arasında istatistiksel fark bulunmazken (p>0.05), oda sıcaklığında 6 ay bekletilme sonucunda bağlanma dayanımının anlamlı derecede daha düşük olduğu gözlendi (p<0.05). Sonuç: Saklama koşulları kompozit restorasyonun dentine bağlanma dayanımını etkilemektedir. Adezivler üretici talimatlarına uygun bir şekilde buzdolabında saklanmalıdır.Anahtar kelimeler: Self-Etch adeziv, makaslama bağlanma dayanımı, nanohibrit kompozit THE EFFECT OF DIFFERENT STORAGE CONDITIONS ON THE DENTIN BOND STRENGTH OF A SELF-ETCH ADHESIVE Aim: This study evaluated the effect of different storage conditions on dentin shear bond strength of a Self-Etch adhesive. Material and Methods: Forty extracted non-caries human molars were used in this study. Flat dentin surfaces were prepared on all teeth and embedded in acrylic resin blocks with the occlusal surfaces facing up. The samples were divided randomly into 5 groups (n=8). A Self-etch adhesive (Clearfil S3 Bond, Kuraray Dental, Okayama, Japan) and a nanohybrid composite resin (Grandio; Voco, Cuxhaven, Almanya) was appli- ed on dentin surface with 3 mm diameter and 4 mm height in a mold. In the first group, composite resin was applied after adhesive and polymerized with LED curing unit (3M Espe, Seefeld, Germany). Samples were stored in distilled water for 24 hours and then shear bond test was applied with a universal testing machine. After this, one of the adhesive was  refrigerated and the other one was stored at room temperature. After 1 month and 6 months each adhe- sive was applied on one group and shear test was performed. Data were recorded and one-way ANOVA test was done with SPSS 20 software (α=0.05). Results: The highest bond strength was observed in samples prepared after 24 hours (18,35 MPa). After 1 month, according to the results, there was no significant difference between samples prepared with the adhesive stored at refrigerated temperature or room temperature (p>0.05), while bond strength values of room temperature after 6 months’ group were significantly lower than the others groups (p<0.05). Conclusion: Storage conditions of bonding adhesives are important factors for the bond strength of composite resins to dentin. The adhesives should be stored in the refrigerator as recommended by the manufacturer’s instructions

___

  • 1. Garbui BU, Botta SB, Reis AF, Matos AB. Comparison of chemical aging and water immersion time on durability of resin-dentin interface produced by an etch-and-rinse adhesive. J Contemp Dent Pract 2012; 13: 464-71.
  • 2. Mutluay MM, Yahyazadehfar M, Ryou H, Majd H, Do D, Arola D. Fatigue of the resin-dentin interface: a new approach for evaluating the durability of dentin bonds. Dent Mater 2013; 29: 437-49.
  • 3. Tay FR, Carvalho RM, Pashley DH. Water movement across bonded dentin - too much of a good thing. J Appl Oral Sci 2004; 12: 12-25.
  • 4. Abdalla AI, El Zohairy AA, Aboushelib MM, Feilzer AJ. Influence of thermal and mechanical load cycling on the microtensile bond strength of self-etching adhesives. Am J Dent 2007; 20: 250-4.
  • 5. Akın GE, Hergüner-Siso Ş, Akın H. Termal Siklus ve Suda Bekletmenin Kendinden Asitli Adezivlerin Dentine Mikrogerilim Bağlanma Dayanımları Üzerine Etkileri. Atatürk Üniv Diş Hek Fak Derg 2012; 22: 125-31.
  • 6. Toledano M, Osorio R, Albaladejo A, Aguilera FS, Osorio E. Differential effect of in vitro degradation on resin-dentin bonds produced by self-etch versus total-etch adhesives. Journal of Biomedical Materials Research Part A 2006; 77: 128-35.
  • 7. Spohr AM, Correr Sobrinho L, Consani S, Sinhoreti MA, Borges GA. Effect of refrigeration on tensile bond strength of three adhesive systems. Braz Dent J 2001; 12: 75-9.
  • 8. Hagge MS, Lindemuth JS, Broome JC, Fox MJ. Effect of refrigeration on shear bond strength of three dentin bonding systems. Am J Dent 1999; 12: 131-3.
  • 9. Poptani B, Gohil KS, Ganjiwale J, Shukla M. Microtensile dentin bond strength of fifth with five seventh-generation dentin bonding agents after thermocycling: An in vitro study. Contemp Clin Dent 2012; 3: 167-71.
  • 10. Moszner N, Salz U, Zimmermann J. Chemical aspects of self-etching enamel-dentin adhesives: A systematic review. Dent Mater 2005; 21: 895-910.
  • 11. Sadr A, Ghasemi A, Shimada Y, Tagami J. Effects of storage time and temprature on the properties of two sel-etching systems. J Dent 2007; 35: 218-25.
  • 12. Nishiyama N, Suzuki K, Yoshida H, Teshima H, Nemoto K. Hydrolytic stability of methacrylamide in acidic aqueous solution. Biomaterials 2004; 25: 965-9.
  • 13. Davis EL, Joynt RB, Yu X, Wieczkowski G, Jr. Dentin bonding system shelf life and bond strength. Am J Dent 1993; 6: 229-31.
  • 14. Hagge MS, Lindemuth JS, Broome JC, Fox MJ. Effect of refrigeration on shear bond strength of three dentin bonding systems. Am J Dent 1999; 12: 131-3.
  • 15. Nishiyama N, Tay FR, Fujita K, Pashley DH, Ikemura K, Hiraishi N, King NM. Hydrolysis of functional monomers in a single-bottle self-etching primer - Correlation of C-13 NMR and TEM findings. J Dent Res 2006; 85: 422-6.
  • 16. Spohr AM, Correr-Sobhirno L, Consani S, Sinhoreti MAC, Borges JA. Effect of refrigeration on tensile bond strength of three adhesive systems. Braz Dental J 2001; 12: 75-9.
  • 17. Borges GA, Spohr AM, de Oliveira WJ, Correr-Sobrinho L, Correr AB, Borges LH. Effect of refrigeration on bond strength of self-etching adhesive systems. Braz Dent J 2006; 17: 186-90.