Funda BAYINDIR, Esra KUL, Ayşe Esin AKTAŞ, Ayşe :BAŞTOPÇU

NİSTATİN VE TİTANYUM DİOKSİT İLAVESİNİN YUMUŞAK ASTAR MATERYALİNDEKİ CANDIDA KOLONİZASYONU ÜZERİNE ETKİSİNİN KARŞILAŞTIRILMASI

Amaç: Tam protez kullanan hastalarda, yumuşak astar materyalleri, mukozayı irrite eden lezyon oluşumları ile sonuçlanan, Candida albicans’ ların adezyonunu COMPARISON OF THE EFFECT OF NYSTATIN AND TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL ABSTRACT ve kolonizasyonunu destekleyebilir. Bu çalışmanın amacı, nistatin ve titanium dioksit [TiO2] antifungal ajanlarının, yumuşak astar maddelerindeki Candida albicans kolonizasyonunu önlemedeki etkisini değerlendirmektir. Gereç ve Yöntem: Ufi Gel P ve Mollosil yumuşak astar materyallerinden 5mm çapında ve 2mm kalınlığında, 5%, 10%, and 15% konsantrasyonda nistatin ve titanyum dioksit içeren diskler hazırlandı. Kontrol grubu için ise nistatin ve TiO2 içermeyen, sadece yumuşak astar maddesinden oluşan diskler hazırlandı. C. albicans’ lar Sabouraud Dekstroz Agar (SDA) kaplı besiyerine inoküle edildi. Hazırlanan diskler besiyerlerine yerleştirildi. 37° C’de inkübe edildi. Çalışmanın ikinci basamağı için yeniden hazırlanan 10% w/v nistatin içeren diksler zamanla antifungal etkinin değerlendirilmesi için farklı süreler (1, 7, 14, and 16 gün) boyunca su içinde bekletildiler. Daha sonra sudan çıkarılıp, C. albicans inoküle edilmiş SDA plağına yerleştirildiler. Her gruba nistatin veya TiO2 içermeyen bir kontrol diski yerleştirildi. Bulgular: Uygulanan varyans analizi sonucu kontrol disklerinde istatistiksel olarak anlamlı bir C. albicans inhibisyonu yoktu (p <0.001). İn vitro olarak nistatin’in toz formunun yumuşak astarlara eklenmesi, C. albicans kolonizasyonunu önledi. Disklerin suda bekleme süresi, inhibisyon derecesini etkiledi. Fakat, yumuşak astarlara TiO2 ilavesi, C. albicans kolonizasyonunu önlemedi. Sonuç: Bu çalışmanın sonucuna göre, in vitro olarak, nistatinin toz formunun yumuşak astarlara ilave edilmesi, C. albicans kolonizasyonunu önledi fakat aynı miktardaki TiO2 tozu eklenmesi etki göstermedi. Anahtar Kelimeler: Nistatin, titanyum dioksit, Candida albicans COMPARISON OF THE EFFECT OF NYSTATIN AND TITANIUM DIOXIDE ADDITION ON CANDIDA COLONIZATION IN SOFT LINING MATERIAL ABSTRACTAim: Soft liners can support the adhesion and colonization of Candida albicans, which can then irritate the mucosa of denture wearers, resulting in the formation of lesions.The goal of this study was to evaluate the efficacy of fungicidal agents, nystatin and titanium dioxide [TiO2], in preventing the colonization by Candida albicans on soft lining materials. Material and methods: Disks of Ufi Gel P and Mollosil cold-curing, soft relining materials with a diameter of 5 mm and thickness of 2 mm were prepared with nystatin and TiO2 powders in a concentration of 5%, 10%, and 15% (w/v). For the control group, only discs not containing nystatin and TiO2 were prepared, which consisted only of soft lining material. C. albicans was inoculated on Sabouraud dextrose agar (SDA)-coated plates. The disks were placed on these plates and incubated aerobically at 37° C. The Ufi Gel P and Mollosil disks, including 10% w/v nystatin, (re-prepared for the second step of the study) were immersed in water for separate intervals (1, 7, 14, and 16 days) to determine the fungicidal action over time. The disks were then removed and placed on SDA plates inoculated with C. albicans. In each group, one control disk without nystatin or TiO2 was included. Results: A univariate analysis of variance test revealed that the activity of C. albicans was not significantly inhibited on the control disks (p < 0.001). The addition of nystatin in powder form to the soft liners prevented the colonization of C. albicans in vitro. The immersion time of the disks plunged in water appeared to affect the degree of inhibition. However, the addition of TiO2 did not prevent colonization by C. albicans of the soft liners.Conclusion: This study showed that the addition of nystatin in powder form to soft liners prevented the colonization of C. albicans in vitro but that the addition of the same amount of TiO2 powder did not. Keywords: Nystatin, titanium dioxide, Candida albicans

Anahtar Kelimeler:

Nistatin, titanyum dioksit

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