The seroprevalence of Borrelia burgdorferi sensu lato and anaplasma phagocytophilum in clinically healthy dogs from Sinop region of Turkey

Amaç. Sinopunda içinde olduğu Türkiyenin Karadeniz bölgesinde Ixodes ricinus türü keneleresıklıkla rastlanmaktadır. Bu çalışmanın amacı, Sinopun sekiz köyünde yaşayan köpeklerdeBorrelia burgdorferi sensu lato (s.l.) and Anaplasma phagocytophilumun seroprevalansınıaraştırmak ve her iki enfeksiyon etkeni arasındaki epidemiyolojik benzerlikleri ortaya koymaktır.Yöntem. Sinopta, 2007 yılının Ağustos ayında klinik olarak sağlıklı görünen 93 köpekten kanörneği alınmıştır. Serum örneklerinde ELISA yöntemiyle B. burgdorferi s.l. ve A.phagocytophilum IgG antikorları araştırılmıştır. Bulgular. 93 köpeğin 26sında (%28,0) B.burgdorferi s.l. ve 28inde (%30,1) A. phagocytophilum IgG antikorları saptanmıştır. 7 köpekte(%7,5) sadece B. burgdorferi s.l., 9 köpekte (%9,7) sadece A. phagocytophilum antikorları ve 19köpekte (%20,4) ise ko-seroprevalans tespit edilmiştir. B. burgdorferi s.l. seropozitifliği gösterenköpeklerde, seronegatif olanlarına göre A. phagocytophilum seropozitifliği daha yüksek bulunmuşolup (X2= 28,90, P= 0,001), her iki enfeksiyon etkenleri arasında pozitif yönde bir korelasyonsaptanmıştır (Phi korelasyon= 0,584, p=,0.001). Sonuç. Sonuç olarak, Sinop ve benzer iklimselbölgelerde, köpekler yüksek derecede B. burgdorferi s.l. ve A. phagocytophilum enfeksiyonu riskialtındadırlar. Ayrıca köpeklerdeki seroprevalans oranları, belirli bir bölgenin endemik durumunungöstergesi olarak insanlar için de önem taşır.

Türkiye nin Sinop yöresinde, klinik olarak sağlıklı görülen köp eklerde Borrelia burgdorf eri sensu lato ve anaplasma phagocytophilum serop revalansı ve her iki enf eksiyon etkeninin epidemiyoloj ik benzerlikleri

Aim. Ixodes ricinus ticks are frequently encountered in the Black Sea Region of Turkey, includingthe province of Sinop. The aim of this study was to investigate the seroprevalence of Borreliaburgdorferi sensu lato (s.l.) and Anaplasma phagocytophilum in dogs living in eight villages ofSinop and to clarify epidemiological similarities between both infectious agents. Methods. InAugust 2007, blood samples were collected from 93 dogs, which appeared clinically healthy.Serum samples were tested by the ELISA method for the presence of B. burgdorferi s.l. and A.phagocytophilum IgG antibodies. Results. B. burgdorferi s.l. IgG antibodies were detected in 26of 93 dogs (28.0%) and A. phagocytophilum IgG antibodies in 28 of them (30.1%). Only B.burgdorferi s.l antibodies were detected in 7 dogs (7.5%), only A. phagocytophilum antibodies in 9dogs (9.7%) and co-seroprevalence was seen in 19 dogs (20.4%). Seroprevalence of A.phagocytophilum was higher in the seropositive dogs to B. burgdorferi s.l. than the group ofseronegative dogs (X2= 28.90, p= 0.001) and there was a positive correlation between bothinfectious agent (Phi coefficient= 0.584, p= 0.001). Conclusion. Dogs of Sinop in Turkey areunder high risk to get infected with B. burgdorferi s.l. and A. phagocytophilum and most probablyin similar climatic regions of the country. Also the seroprevalence rates in dogs are important to bean indicator of endemic status of a given region for humans, too.

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