Ahmet Sevki TASKIRAN, Erkan GUMUS, Handan GUNES, Ercan OZDEMİR, Gokhan ARSLAN, Hacer Aybike TOPTAS, Özge GÜNEY, Rukiye KUTLU

Siklooksijenaz-2 inhibitörü tenoksikam'ın pentilentetrazol ile oluşturulan epileptik nöbetler üzerine etkisi

Amaç: Epilepsi merkezi sinir sisteminde bir grup nöronun ani, anormal ve hipersenkronize deşarjları ile nöbetler halinde gözlenen beyin fonksiyonlarının kısa süreli paroksimal rahatsızlığı olarak tanımlanır. Nonsteroid anti inflamatuarlardan olan tenoksikam araşidonik asidi prostaglandinlere dönüştüren tip 2 siklooksijenaz (COX2) enzimini selektif olarak inhibe eden kimyasal bir ajandır. Bu çalışmanın amacı siklooksijenaz-2 inhibitörü tenoksikam'ın pentilentetrazol ile oluşturulan epileptik nöbetlere üzerine etkisini araştırmaktır. Yöntem: Çalışmamızda 18 tane 220-240 gr Wistar Albino erkek sıçan kullanılmıştır. Hayvanlar kontrol (n=6), 10 mg/kg/gün tenoksikam (n=6) ve 20 mg/kg/gün tenoksikam (n=6) olmak üzere üç gruba ayrıldı. On gün süre ile kontrol grubuna çözücü ve diğer iki gruba belirtilen dozlarda tenoksikam intramusküler olarak uygulandı. Onuncu gün ilaç uygulamalarından 45 dk sonra pentilentetrazol (PTZ) 70 mg/kg intraperitoneal olarak enjekte edildi. Hayvanlar 30 dk boyunca gözlemlendi. Racine nöbet skalasına göre evreleri belirlendi ve ilk miyoklonik jerk zamanı (FMJ) saniye olarak kaydedildi. İşlem bitiminden sonra hayvanların beyin dokuları alındı. Beyin dokuları rutin histolojik takip sonrası toluidin blue boyası ile boyanandı. Hipokampüste CA1 ve dentat girus bölgelerinde nöronal hasarı gösteren 'Dark nöron' sayıları yüzde olarak belirlendi. Bulgular: Epileptik davranış sonuçları Racine nöbet skalasına (RC) göre değerlendirildiğinde, 10 mg/kg tenoksikam kontrole göre nöbet evresini anlamlı olarak azalttı (p

Effect of the cyclooxygenase-2 inhibitor tenoxicam on pentylenetetrazole-induced epileptic seizures in rats

Objective: Epilepsy is a short-term paroxysmal disturbances of brain functions observed between sudden, abnormal and hypersynchronization discharges and seizures of a group of neurons in the central nervous system. The nonsteroidal anti-inflammatory tenoxicam is chemical agent that selectively inhibits type 2 cyclooxygenase (COX2), which converts arachidonic acid to prostaglandins (PGs). The aim of this study was to investigate the effect of the cyclooxygenase-2 inhibitor tenoxicam on pentylenetetrazole on epileptic seizures. Method: Eighteen Wistar Albino male rats (220±20 g) were divided into three groups: control (n=6), 10 mg/kg/day tenoxicam (n=6) and, 20 mg/kg/day tenoxicam (n=6). Tenoxicam was administered intramuscularly for ten days. On the tenth day, pentylenetetrazol (PTZ) was injected intraperitoneally at 70 mg/kg after 45 minutes of drug administration and the animals were observed for 30 min. Stages were determined according to the Racine seizure scale (RC) and the first myoclonic jerk time (FMJ) was recorded in seconds. After completing procedure, whole brain tissues were removed and stained with toluidine blue stain. The number of dark neurons with chromatin aggregation Results: Epileptic behavior were evaluated according to the RC, 10 mg/kg of tenoxicam significantly reduced the seizure stage compared to the control (p

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