Mustafa Yavuz KÖKER, Hüseyin AVCILAR, Bülent ESER

Sağlıklı kemik iliği ve kan örneklerinde akım sitometri uygulamaları

Akım sitometrik analiz ile daha doğru sonuçlara ulaşabilmek için kemik iliği ve periferik kanhücrelerinin fiziksel morfolojisinin iyi bilinmesi gereklidir. Akım sitometride; FS, SS ve CD45kapısı ile kemik iliği hücresel bileşenleri ve onların karakterleri hakkında iyi bir bilgi edinmekmümkündür. Normal kemik iliği yapısını anlamak, patolojik durumların tanımlanmasında oldukçaönemlidir. Sonuç olarak, sağlıklı kemik iliği ve kan örneklerinin akım sitometri ile doğrutanıyabilmek bizim daha iyi immün-fenotiplendirme yapmamızı sağlar.

Flow cytometry application in normal bone marrow and peripheral blood

The physical morphology of bone marrow and peripheral blood cells has to be well known toachieve more accurate results with flow cytometric analysis. In flow cytometry; FS, SS andthrough the gate of CD45, it is possible to obtain a good knowledge about the characteristic ofbone marrow cellular components. it is very important to understand the structure of normal bonemarrow for the identification of pathological conditions. As a result, to recognize healthy bonemarrow and blood samples correctly by flow cytometry enables us to make better immuno- phenotyping.

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