Merve ERGÜL, Ahmet ALTUN, Mustafa ERGÜL, Mesut PARLAK, Ali Kemal FİLİZ, Tijen KAYA TEMİZ

The Effects of Daily Repeated Magnetic Field on S-Nitroso-N- acetyl-DL penicillamine Induced Hyperalgesia

Amaç. Ağrı tedavisi tıbbın en önemli hedeflerinden biridir. Bu araştırmada manyetik alanuygulamasının S-Nitroso N-acetyl penicillamine (SNAP) tarafından oluşturulan ağrı üzerindekietkilerini ve etki mekanizmasını araştırmayı amaçladık. Yöntem. Çalışma iki kısım olacak şekildetasarlandı. Birindi kısımda hayvanlar her birinde altı hayvan olacak şekilde 4 gruba bölündü.Birinci grup sham grubu olarak belirlendi ve bu hayvanlara 0,3 mL %0,9 NaCl tail flick latensleriölçülmeden once uygulandı. İkinci gruba (SNAP grubu) 2 mg/kg SNAP i.p. olarak enjekte edildive sham grubu ile aynı zaman noktalarında tail flick ölçümleri yapıldı. Üçüncü grup (MF grubu)tekrarlayan altı gün boyunca manyetik alana maruz bırakıldı. Dördüncü grupta (SNAP+MF)hayvanlara manyetik alan uygulaması yapılmadan hemen önce 2 mg/kg SNAP i.p. olarakuygulandı. Çalışmanın ikinci kısmında ratlardan kan örnekleri alınarak kanlarında supstance p veB-endorfin seviyelerine ELISA yöntemi ile bakıldı. Bulgular. SNAP (2 mg/kg) anlamlı birhiperaljezi meydana getirerek tail flick latenleslerini kısalttı. Manyeteik alan uygulaması (5 mT ve165 dakika/gün) özellikle 3. ve 4. günlerde anlamlı bir analjezi meydana getirdi. SNAP grubundasubstance p seviyeleri yüksek bulunurken, manyetik alan grubunda B-endorfin seviyelerininyüksek olduğu tespit edildi. Sonuç. Bu sonuçlar manyetik alan uygulamasının ağrı tedavisindealternatif bir yaklaşım olabileceğini göstermektedir. Fakat bu yöntemin kullanılabilmesi içingelişen toleransın üstesinden gelmenin yollarının bulunması ve bunun içinde ileri araştırmalaragereksinim vardır.

Günlük olarak tekrarlanan manyetik alan uygulamasının-Nitroso-N-acetyl-DL p enicillamine taraf ından indüklenen hip eralj ezi üzerine olan etkisi

Aim. The treatment of pain has been one of the most important objectives of medicine. We aimedto investigate antinociceptive effects and mechanisms of magnetic field (MF) on the hyperalgesiaproduced by S-Nitroso N-acetyl penicillamine (SNAP). Method. Study has been made in twosections. In the first section, rats were divided four groups (six in each). The first group wasdetermined as sham group and administrated 0.3 mL, 0.9% NaCl intraperitoneally (i.p) beforeassessing tail flick latencies (TFLs) (Sham group). In the second group, 2 mg/kg SNAPadministrated i.p. and TFLs were assessed at the same time points with first group (SNAP group).In the third group, rats were repeatedly exposed to MF for 6 consecutive days (MF group). In thefourth group, SNAP was administrated i.p everyday shortly before MF exposure (SNAP+MFgroup). In the second section, animals divided to the same groups with the first section. Sameprocedures have been performed with the first section groups and blood samples were collected todetermine plasma levels of β-endorphin and substance P. Results. SNAP (2 mg/kg) producedhyperalgesic effect with i.p. administration. MF application (5 mT and 165 min per day) produceda strong antinociception in Days 3 and 4. Tail flick values of SNAP+MF in Days 3 and 4 werefound to be significantly low as compared to MF and Sham groups. In SNAP group, substance Plevels were found to be significantly high. Plasma β-endorphin levels in MF and SNAP+MFgroups were significantly high as compared to the Sham group. Conclusion. MF may be analternative antinociceptive approach for pain treatment. There is need for further studies toovercome the tolerance to antinociceptive effects of MF.

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