Kemikle yer değiştirebilir α-Al2O3 malzemelerin mikro yapısal değerlendirmesi ve sitotoksik incelemeleri

Amaç: α-Al2O3’nın alçı kalıba döküm yöntemiyle gözenekli olarak üretilebilirliğinin değerlendirilmesi, MTT ve Agar difüzyon testleriyle sitotoksisitesinin ve SEM ile de mikroyapısal incelemesinin yapılması. Yöntem: Gözenekli ve doku büyüyebilir α-Al2O3 üretimi için alçı kalıba döküm yöntemi kullanılmıştır. Morfolojik analiz için SEM kullanılmıştır. Sitotoksisite için MTT canlılık testi ve Agar difüzyon testi kullanılmıştır. Bulgular: Yapılan testler, α-Al2O3’nın positif kontrol grubuna yakın canlılık değerleri verdiğini göstermiştir. Bu da aluminanın kemik ile yer değiştirebilir bir aday malzeme olabileceğini göstermektedir. Sonuç: Gözenekli alumina üretimi başarılı olmuştur. Özellikle sitotoksisite testleri göstermiştir ki alumina kemikle yer değiştirebilir çok önemli ve uygun bir adaydır.

Microstructural evaluation and cytotoxicity investigations of α-Al2O3 bone replacement materials

Objective: To evaluate the production of porous α-Al2O3 by slip casting method and to investigate the cytotoxicity byMTT and Agar diffusion tests as well as SEM microstructural evaluation.Method: Ceramic process route includes slip casting for porous osteoconductive α-Al2O3 production. For examination ofmorphology, SEM was employed. To evaluate the cytotoxicity, MTT and Agar diffusion tests were conducted.Results: The conducted tests have given very close results to positive control groups which mean that the product αAl2O3 is a very good candidate for bone replacement and osteoconductivity.Conclusions: The porous α-Al2O3 production was succeeded and the cytotoxicity tests have been approved that thismaterial can be a good candidate for replacement.

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