Necati ÖZPINAR, Semra ÖZÇELİK, Önder YÜNLÜ

Isolation and morphotyping of Acanthamoeba spp. and Vermamoeba spp. from hospital air- conditioning systems

Amaç: Çalışmada, Cumhuriyet Üniversitesi Araştırma ve Uygulama Hastanesi klimalarında Acanthamoeba ve Vermamoeba gibi serbest yaşayan amiplerin (SYA) varlığının araştırılması amaçlanmıştır. Yöntem: Bu amaçla, hastanenin beş farklı ünitesinden toplam 24 klimanın ızgara ve filtre kısımlarından steril eküvyonlarla sürüntü örnekleri alınmıştır. Alınan sürüntü örnekleri steril tüplerde laboratuara getirilmiş ve üzerine inaktive edilmiş E.coli içeren Besleyici Değeri olmayan Agar plaklarına yayılmıştır. Ekim yapılan plaklar 300C de inkübe edilmiştir. Üreme olan plaklardan pasajlar yapılmıştır. Saptanan amipler morfotiplendirme anahtarları kullanılarak cins düzeyinde tanımlanmıştır. Bulgular: İncelenen 24 klima örneğinin 14'ünde (%58.3) SYA saptanmıştır. Bunlardan 10'unda (%41.6) Vermamoeba spp, 4'ünde (%16.7) Acanthamoeba spp. tanımlanmıştır. Sonuç: Bu bulgular klima sistemlerinde potansiyel patojen SYA ler açısından dikkatli olunması gerektiğini göstermektedir. Ayrıca, bu amipler içlerinde patojen bakteri, mantar ve virusları taşıyabilmektedir. Hem SYA hem de içlerinde taşıyabilecekleri endosimbiyontlarıyla ilgili riskleri azaltmak için havalandırma sistemleri düzenli olarak etkili dezenfektenlarla temizlenmelidir

Hastane klima sistemlerinden Acanthamoeba ve Vermamoeba türlerinin izolasyonu ve morfotiplendirilmesi

Objective: The aim of the study was to investigate the presence of free-living amoebae (FLA) such as Acanthamoeba and Vermamoeba in air conditioning systems of Cumhuriyet University Research and Application Hospital (CURAH). Method: For this purpose, twenty-four dust samples were collected using sterile cotton swaps from grilles and filters of five different unit air-conditioners of CURAH located in Sivas, Turkey. Samples were transferred in a sterile tubes to the laboratory and spread onto non-nutrient agar (NNA) plates with a lawn of inactive Escherichia coli. Culture plates were incubated at 30oC. FLA genera was identified according to morphotyping keys. Results: FLA were found in 14 (58.3%) of examined 24 air-conditioner. Ten (41.6%)were identified as Vermamoeba spp, 4 (16.7%) as Acanthamoeba spp., after microscobic examination. Conclusions: These findings should alert us on the risk of Acanthamoeba and Vermamoeba infections. In addition, these amoebas could be host for bacteria, fungi and viruses and indirectly become agents of transmission for these pathogens. For decrease the risk both Acanthamoeba and their endosymbionts, regularly cleaning of air-conditioner filters and grilles using effective disinfectants are essential

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