Serpil SARAYDIN ÜNVER, Zeynep Deniz İNAN ŞAHİN

Histological approaches to determination of stem cells in neural tube defects which are created experimentally

Kök hücreler gelişimin erken dönemlerinde görülmeye başlayan, hem sinir hem de diğer sistemlerin gelişiminde önemli rol alan hücrelerdendir. Nöral tüpün kapanmamasıyla oluşan anomaliler nöral tüp defektleri olarak bilinir. Nöral tüp defekti oluşumu, nöral tüpün gelişmeye başladığı andan itibaren pek çok mekanizma ve gen bölgesinin işleyiş bozukluğuna bağlı olabilir. Dünyada ve ülkemizde nöral tüp defekti insidansı oldukça yüksektir. Bu nedenle insanlarda meydana gelen nöral tüp defektini anlamak için genellikle deney hayvanlarına teratojen ajan verilerek gerekli bilgiler toplanır. Bu derlemeyle deneysel nöral tüp defekti oluşturulmuş ve defektin oluşumuda rol oynadığı düşünülen bazı kök hücrelerin immünhistokimya ve immünflorasan çalışmaları derlenmiştir. Buradan hareketle, kök hücre belirteçlerinden faydalanarak nöral tüpün normal gelişim ve defekt oluşum sürecindeki dağılımlarını belirlemek, kök hücrelerin çoğalma ve farklanma mekanizmalarını anlamayı ve beraberinde defekt patogenezinin ortaya çıkarılmasına katkı sağlayabilmesi hedeflenmiştir.

Deneysel olarak oluşturulan nöral tüp defektlerinde kök hücrelerin belirlenmesi konusunda histolojik yaklaşımlar

Stem cells are cells that are started to be seen in the early stages of development and they have important roles in development of both neural system and other systems. Anomalies that occur with the neural tubes inability to close are known as neural tube defects. Neural tube defects can be due to malfunction of gene area and many mechanisms since the moment neural tube starts to develop. The incidence of neural tube defect is very high in our country and in the world. Therefore, to understand the neural tube defects in the people, necessary data is usually collected by giving teratogen agent to subject animals. With this compilation, experimental neural tube defects have been formed and immunohistochemistry and immunofluorescent studies of some stem cells that are thought to have role in the formation of defect are compiled. From this point of view, it is aimed to determine the distributions of neural tube in rhe processes of normal development and defect formation, to understand the reproduction and alteration mechanisms of stem cells, and concomitantly to reveal the defect pathogenesis.

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