Mehmet ALAKAYA, Elvan Çağlar ÇITAK, Simge TAŞKINLAR, Sakir Necat YILMAZ, Ahmet Ata ÖZÇİMEN, Mehmet Yavuz Burak ÇİMEN

Glutamin ve β-hidroksi β-metil bütiratin metotreksatin indüklediği intestinal mukozit üzerine etkisi

Amaç: Metotreksatın sebep olduğu intestinal mukozit üzerinde Glutamin (Gln) ve β-hidroksi β-metilbutiratın (HMB) önleyici etkilerini değerlendirmeyi amaçladıkGereç ve Yöntem: 50 Wistar albino cinsi sıçan 5 gruba (G) ayrıldı. G1 kontrol grubu ve G2 metotreksat (MTX) ile tedavi edilen gruptu. G3, G4 ve G5 sırasıyla MTX ve Gln, Mtx ve HMB, MTX ve Gln, HMB verilen gruplardı. Sıçanlara üç gün intraperitoneal MTX uygulandı. Beşinci günde bağırsak doku örnekleri alındı.Bulgular: G5 kript derinlik ölçümleri G1 grubu ile benzerdi. Park skorlamasına göre G1 ve G5, beş çalışma grubu arasında en yüksek skoru alan gruplardı. Doku kaspaz-3 ile incelendiğinde, en düşük apoptotik oran G5'te bulundu.Sonuç: Bu çalışma, Gln ve HMB kullanım kombinasyonunun her iki kimyasal maddenin ayrı kullanımından daha etkili olduğunu göstermiştir

Anahtar Kelimeler:

Glutamin, β-hidroksi β-metilbutirat, metotreksat, rat, mukozit

Effects of glutamine and β-hydroxy β-methyl butyrate on methotrexate induced intestinal mucositis

Purpose: We aimed to evaluate preventing effects of Glutamine (Gln) and β-hydroxy β-methylbutyrate (HMB) on methotrexate induced intestinal mucositisMaterials and Methods: Fifty Wistar albino rats were divided into 5 groups (G). Group G1 defined as control and G2 was the methotrexate (MTX) treated group. The group G3, G4 and G5 were MTX plus Gln, Mtx plus HMB, MTX plus Gln and HMB respectively. Distilled water was applied by gavage to the first 2 groups. Rat received intraperitoneal injections of MTX at the third day. On the fifth day intestinal tissue samples were obtained. Results: The G5 had similar results with the G1 group in the crypt length. According to Park scoring, G1 and G5 were both the highest scores among five study groups. When the tissue was inspected by caspase-3 coating, the lowest apoptotic rate is found in the G5. Conclusion: This research showed that the combination of Gln and HMB use is more effective then the separate use of both chemicals.

Keywords:

Glutamine, β-hydroxy β-methylbutyrate, mucositis, methotrexate, rat,

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