Fatma DAĞLI, İnayet GÜNTÜRK, Gönül Şeyda SEYDEL, Cevat YAZICI

Deneysel hipertiroidide fiziksel ve vital bulguların ve karnozinin etkisinin değerlendirilmesi

Amaç: Bu çalışmada, deneysel hipertiroidinin ve antioksidan özelliği bilinen karnozinin ratların fiziksel ve vital bulgular üzerine etkilerinin araştırılması; ve bu parametreler ile serbest T3 (FT3) seviyeleri arasındaki ilişkinin belirlenmesi amaçlandı. Gereç ve Yöntem: Ratlar; her biri 12 rattan oluşan (n=12) kontrol (CONT), hipertiroidi 1 (T), hipertiroidi 2 (T-T), Hipertiroidi-1 + Karnozin (T-C), Hipertiroidi-2 + Karnozin (T-TC), Karnozin (C) ve Karnozin + Hipertiroidi-1 (C-T) olmak üzere yedi gruba ayrıldı. Ratlarda hipertiroidi modeli oluşturmak için intraperitoneal (ip) olarak rat ağırlığı/gün başına 300 µg/kg L-tiroksin (L-T4) ve karnozinin etkilerinin belirlenmesi için rat ağırlığı/gün başına 300 µg/kg dozunda karnozin (ip) uygulandı. Bulgular: 10 ve 20 günlük tiroksin uygulaması ile FT3 düzeylerinin (T:3.640.51pg/mL, T-T: 4.060.91pg/mL) ve vücut sıcaklığının (T:37.10.3oC, T-T: 37.60.3oC) anlamlı şekilde arttığı, vücut ağırlığının (T:240.722.0g, T-T:263.028.7g) azaldığı tespit edildi. Karnozin uygulamasının etkileri incelendiğinde, sadece profilaktik uygulamada FT3 düzeylerinin yükselmesini engellediği, diğer parametreler üzerinde iyileştirici etkisinin olmadığı görüldü. Sonuç: L-T4 uygulamasıyla gözlenen artmış FT3 düzeylerinin elde edilen fiziksel ve vital bulgularla uyumlu olduğu ancak karnozin uygulamasının hipertiroidik tabloda gözlenen fiziksel bulgular üzerinde beklenen etkileri yansıtmadığı tespit edildi.

Evaluation of physical and vital signs and the effect of carnosine in experimental hyperthyroidism

Purpose: This study aims to investigate the effects of experimental hyperthyroidism and carnosine which is known to have antioxidant properties on physical and vital findings in rats, and to determine the relationship between these parameters and free T3 (FT3) levels. Materials and Methods: Rats were analyzed in 7 groups (each containing 12 animals); control (CONT), hyperthyroidism-1 (T:10-day L-thyroxine (L-T4) administration), hyperthyroidism-2 (T-T: 20-day L-T4 administration), Carnosine (10 day carnosine administration), Hyperthyroidism-1 + Carnosine (T-C), Hyperthyroidism-2 + Carnosine (T-TC), and Carnosine + Hyperthyroidism-1 (C-T). In order to create a hyperthyroidism model, L-thyroxine (L-T4) doses of 300 µg/kg rat weight/day and carnosine doses of 300 µg/kg rat weight/ day were intraperitoneally (ip) administered to the rats. Results: After 10 and 20 days of thyroxine administration, FT3 levels (T:3.640.51pg/mL, T-T: 4.060.91pg/mL) and body temperature (T:37.10.3oC, T-T: 37.60.3oC), significantly increased while body weight decreased (T:240.722.0g, T-T:263.028.7g). Carnosine administration only prevented the increase of FT3 levels, but had no effect on other parameters. Conclusion: The increased FT3 levels observed with L-T4 administration were consistent with the physical and vital findings, but carnosine administration did not reflect the expected effects on the physical findings observed in the hyperthyroid condition.

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