D-pantotenik asidin PC-3 prostat kanseri hücreleri üzerindeki apoptotik etkilerinin incelenmesi

Amaç: D-pantotenik asidin anti-enflamatuar ve antioksidan özellikleri gösterilmiştir ve dekspentanolün hücre ölümünü tetikleyen enflamatuar yolaklar ve apopototik yollakar üzerindeki etkileri, merak uyandırmaktadır. Apoptotik yolaklar, kanser hastalıklarında ve kanser gelişiminde kemoterapötiklere karşı dirençlilik açısından önemlidir. Bu nedenle, PC-3 insan prostat kanseri hücrelerinin dekspantenol ile tedavisinde apoptotik ve inflamasyon mediyatörlerinin seviyelerini ve aktivitelerini nasıl etkileyeceğini planladık. Bu amaçla, insan prostat kanseri hücre kültürü yapıldı. Gereç ve Yöntem: İnsan prostat kanseri hücreleri, dekspentanaol ile muamele edildi ve hücrelerde gadd153, AIF, grp78, bax ve bcl-2 gibi enflamatuar ve apoptotik yolaklardaki aracıların protein seviyeleri ve aktiviteleri ELISA ile analiz edildi. Bulgular: Çalışmamızın sonuçları, D-pantotenik asidin PC-3 prostat kanseri hücrelerinde bax, bcl-2 ve grp78 protein ekspresyonu düzeylerini istatistiksel olarak azaltmadığını gösterdi. PC-3 hücrelerinde D-pantotenik asidin üzerindeki etkisi, gadd153 ve AIF proteinleri arttırıldığı, ancak bu artış seviyesi istatistiksel olarak anlamlı değildi. Sonuç: Son çalışmalar, anti-enflamatuar ilaçların potansiyel faydalarını göstermiştir. Çalışmamız D-pantotenik asidin PC-3 hücrelerinin büyümesi ve hücre içi apoptotik yolaklar üzerinde anlamlı bir etkisinin olmadığını göstermiştir.

Anahtar Kelimeler:

D-pantothenic acid, dexpanthenol, prostat kanseri

Investigation of apoptotic effects of D-pantothenic acid on PC-3 prostate cancer cells

Purpose: The anti-inflammatory and antioxidant properties of D-pantothenic acid have been demonstrated and the effects of dexpentanol on inflammatory pathways and apopototic pathways that trigger cell death are of interest. Apoptotic pathways are important in resistance to chemotherapeutics in cancer diseases and in cancer development. Therefore, we planned how treatment of PC-3 human prostate cancer cells with dexpanthenol will affect the levels and activities of apoptotic and inflammation mediators. For this purpose, human prostate cancer cell culture was performed. Materials and Methods: The human prostate cancer cells were treated with dexpentanaol then protein levels and activities of inflammatory and apoptotic pathway mediators such as gadd153, AIF, grp78, bax and bcl-2 in the cells were analyzed by ELISA. Results: The results of our study showed that, D-pantothenic acid did not statisticaly decreased the leves of bax, bcl-2 and grp78 protein expression in PC-3 prostate cancer cells. The effect of D-pantothenic acid on gadd153 and AIF proteins in PC-3 cells was increased but this increased level did not statisticaly significant. Conclusion: Recent studies have demonstrated the potential benefits of anti-inflammatory drugs. Our study showed that D-pantothenic acid had no significant effect on the growth of PC-3 cells and has no significant effect on intracellular apoptotic pathways.

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