Floretin ve floridzin'in farelerde indometazine bağlı gelişen mide ülserine karşı koruyucu etkileri: potansiyel moleküler mekanizmaların karakterizasyonu

Amaç: Bu çalışmada farelerde indometazine bağlı ülser modelinde, floretin ve floridzinin potansiyel koruyucu etkilerini incelemeyi amaçlanmıştır. Gereç ve Yöntem: Toplam 54 dişi Balb/C faresi dokuz gruba ayrıldı. Famotidin standart anti-ülser madde olarak kullanıldı. Floretin ve floridzin, tedavi gruplarına 50 ve 100 mg/kg dozlarında verildi. Deneysel prosedürlerden sonra, mide dokusunda oksidatif parametreleri (SOD, GSH ve MDA), enflamatuar sitokin TNF-α ve COX1 ve COX2 mRNA ekspresyonları analiz edildi. Ayrıca, floretin ve floridzinin anti-ülser etki mekanizmasını açıklığa kavuşturmak için ülserli alanların sayısal yoğunlukları analiz edilmiştir. Bulgular: Floretin ve floridzin, indometazine bağlı gelişen ülser oluşumunu doza bağımlı olarak azalttı. İndometazin uygulamasından sonra enflamasyon ve oksidatif stresin arttığı belirlendi. Floretin ve floridzin tedavisi indometazin ile tedavi edilen gruba kıyasla tüm parametreleri normalleştirmiştir. Tedavilerin ardından, indomethazin grubuna kıyasla, SOD aktiviteleri ve GSH düzeyleri yükselirken, MDA düzeyleri azalmıştır. Floretin ve floridzin uygulamarının TNF-α, COX1 ve COX2 mRNA ekspresyonlarını azalttığı tespit edildi. Sonuç: Sonuçlarımız oksidatif stres ve enflamasyona karşı potansiyel düzenleyici etkileri nedeniyle floretin ve floridzinin peptik ülser hastalığı için alternatif bir tedavi olabileceğini göstermiştir.

Anahtar Kelimeler:

Phloretin, Phloridzin, Ulcer, Mice

Protective effects of phloretin and phloridzin on indomethacin-induced gastric ulcers in mice: characterization of potential molecular mechanisms

Purpose: We aimed to examine the potential protective effects of phloretin and phloridzin in indomethacin induced ulcer model in mice. Materials and Methods: In total 54 female Balb/C mice were separated into nine groups. Famotidine was used as standard antiulcer agent. The phloretin and phloridzin was given at the doses of 50 and 100 mg/kg as a pre-treatment. After experimental procedures stomach tissue oxidative parameters (SOD, GSH and MDA), inflammatory cytokine TNF-α, and COX1 and COX2 mRNA expressions were analyzed. In addition, to clarify antiulcer effect mechanism of phloretin and phloridzin, numerical densities of ulcerative areas were analyzed. Results: Phloretin and phloridzin inhibited indomethacin-induced ulcer formation in dose dependent manner. Tissue inflammation and oxidative stress were increased after the indomethacin administration. Phloretin and phloridzin treatment normalized all parameters compared to indomethacin treated group. After the treatments, SOD activities and GSH levels were increased while MDA levels were decreased. Phloretin and phloridzin treatments decreased TNF-α, COX1 and COX2 mRNA expressions. Conclusion: Our results showed that phloretin and phloridzin may be an alternative treatment for peptic ulcer disease due to their potential regulatory effects against oxidative stress and inflammation.

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