Midye ile beslenen sıçanların böbrek dokusundaki histopatolojik değişikliklerin immünohistokimyasal yöntemle incelenmesi

Amaç: Bu çalışmada denizlerin önemli filtre işlevini gören midyelerin sıçan böbrek dokusu meydana getirdiği histopatolojik değişikleri belirlemeyi amaçladık. Gereç ve Yöntem: Çalışmamızda Wistar albino cinsi 24 adet dişi sıçan (200-260 g) kullanıldı. Birinci grup (n:6): standart sıçan yemi ile beslenen, ikinci grup (n:6): 4/5 midye + 1/5 standart sıçan yemi ile her gün; üçüncü grup (n:6): 4/5 midye + 1/5 standart sıçan yemi ile gün aşırı; dördüncü grup (n:6): 4/5 midye + 1/5 standart sıçan yemi ile her üç günde bir verilecek şekilde gruplar oluşturuldu. Denek ve kontrol gruplarından alınan tüm böbrek doku örnekleri rutin histopatolojik takip yapıldıktan sonra Hematoksilen- Eozin boyamasıyla, nefrotoksik etki ise immünohistokimyasal olarak TGF-β ve NF-κB ile boyanarak ışık mikroskopta görüntü analiz sistemi ile analiz edildi. Bulgular: Midye ile beslenen sıçanların böbrek dokusunda mononükleer hücre infltrasyonuna, distal ve proksimal tübüllerde dilatasyona, glomerüllerde büzüşmelere ve tübül epitelinde dejenerasyona neden olduğu görülmüştür. İmmünohistokimyasal boyamlarda da TGF-β ve NF-κB immünreaktivitesinin inflamasyona ve apoptoza giden hücrelere bağlı arttığı tespit edilmiştir. Özellikle her gün midye verilen sıçanlarda nefrotoksik etkinin oldukça fazla olduğu görüldü. Sonuç: Sağlıksız koşullarda temin edilen midyelerin fazla tüketimine bağlı boşaltım sistemi organlarından böbrek dokusunda kronikleşebilecek hasarlar oluşturabileceği gösterilmiştir.

Immunohistochemical investigation of histopathological changes in the kidney tissue of mussel-fed rats

Purpose: The aim of this study was to determine the histopathological changes that the mussels, which serve as important filters of the sea, cause in rat kidney tissue. Materials and Methods: In our study, 24 female (Wistar albino 6-10 week old rats) rats were used. First group (n: 6): fed with standard rat food, second group (n: 6): fed everyday with 4/5 mussels + 1/5 standard rat food; third group (n: 6): fed 4/5 mussels + 1/5 standard rat food every two days; Fourth group (n: 6): were formed with 4/5 mussels + 1/5 standard rat food to be given every three days. After the routine histopathological follow-up, all kidney tissue samples taken and control groups were analyzed with Hematoxylin-Eosin staining, and the nephrotoxic effect was immunoshistochemically with TGF-β and NF-κB and analyzed with a light microscope image analysis system. Results: Mussel-fed rats were found to cause inflammation in the kidney tissue, dilatation in the distal and proximal tubules, shrinkage in the glomeruli and degeneration in the tubular epithelium. In immunohistochemical staining, TGF-β and NF-κB immunoreactivity increased due to cells leading to inflammation and apoptosis. The nephrotoxic effect was quite high especially in rats given daily mussels. Conclusion: The mussel provided in unsanitary conditions may cause damage in kidney tissue from the excretory system organs due to excess consumption.

Keywords:

Mussel, Immunohistochemistry, Kidney Histophatology, Nephrotoxicity,

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