Gebeliğin farklı evrelerinde NK (CD56) ve IFN-γ etkileşimi üzerine farklı dozlardaki alkolün etkisi

Amaç: Gebelikte maternal reddin egellenmesi, immün toleransın gelişmesi inhibitör veya stimülatör faktörlerin etkisiyle olmaktadır. Gebeliğin devamı, immünsupresyon ile sağlanmaktadır. Bu sureçte teratojenik faktörler direkt fetusu etkileyerek tolerans mekanizmasını değiştirmektedir. Araştırmamızda, gebeliğin farklı evrelerinde, farklı dozda alkolün hem doğal hem de spesifik immün sistemin kilit hücreleri olan NK(CD56) ve sitokinleri ile etkileşimini incelemeyi planladık. Yöntem: Çalışmamızda 60 wistar albino soyu dişi sıçanla 6 çalışma grubu oluşturuldu. (Kontrol grubu, %17.5 diyet etanol uygulanan grup, %30 gavaj etanol uygulanan grup, kontrol gebe, %17.5 diyet etanol uygulanan gebe, %30 gavaj etanol uygulanan gebe). NK (CD56); Antikandidal İndeks Tayin Metodu, CD19, IL-2r (CD25), Flow Sitometrik Yöntemiyle ve IL-1, IL-2, IFN-g, ELISA yöntemiyle tayin edildi.Bulgular: Bulgularımızda gebeliğin 3. evresinde (17. gün) özellikle gavaj etanol uygulanan gruplarda güçlü supresyonun olduğunu gösterdik.Sonuç: Sonuç olarak, direkt ve indirekt uyguladığımız etanolün supressif etkisinin gebeliğin ileri evresinde güçlü olduğu ve uygulanan alkolün bu dozların kritik seviyelerde olduğunu bulduk. Daha yüksek dozda alkolün abortus riskini arttıracağını ve fetal anomaliyi arttıracağı yönünde bulgular bu düşünceyi güçlendirmektedir.

The interaction of alcohol at various doses with NK and IFN-gamma at each pregnancy stage

Objectives: During pregnancy, the factors affecting maternal rejection are immune tolerance development inhibitors or stimulators. Pregnancy progresses as a result of immunosuppression. Teratogenic factors which directly affect the fetus alter tolerance mechanisms. This study examined the interaction effect of alcohol at different dose rates with both natural and specific lock NK(CD56) and cytokines at various stages of pregnancy.Methods: In this study 60 female Wistar albino rats were randomly assigned to 6 groups: For non pregnant and pregnant rats there were three groups (a) controls, (b) control pregnant (c) alcohol 17.5% of diet, (d)pregnant rats treated similarly (e) alcohol 30% by gavage (f) pregnant group received ethanol through gavage NK(CD56) levels were determined by the Anticandidal Index Determination Method, CD 19 and IL-2r(CD25) by the Flow Cytometric Method and IL-1, IL-2 and IFN-g; by the ELISA method.Results: Suppression at (the advanced stage of pregrancy) stage 3 (17 days) in the both alcohol groups was markedly higher than controls and suppression in the 30% alcohol group significantly higher than in the 17.5% group.Conclusion: This is supported by findings that higher doses of alcohol increase the risk of abortion and fetal abnormalities.

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