Pinar AKSU KILIÇLE, Evren KOÇ, Abdullah DOĞAN, Süleyman GÜL, Ali Nazmi Can DOĞAN, Yusuf ERSAN

Investigation of the Effect of Acrylamide on Capoeta Capoeta (Guldensttead 1773) by Histopathological, Electrophoretic and Biochemical Methods

The aim of this study was to investigate the effects of acrylamide on Capoeta capoeta (Guldensttead 1773) by histopathological, electrophoretic and biochemical methods. Capoeta capoeta caught from Kars stream were used in the study. The fish were divided into 5 groups, each containing 10 pieces, and placed in 300 liter tanks with tap water. Group 1 was kept as a negative control. 20 mg / kg cyclophosphamide given to group 2 (i.p. positive control group), 10 mg / L acrylamide given to group 3, 20 mg / L acrylamide given to group 4 and 30 mg / L acrylamide given to group 5. After all groups were kept in tanks for 4 days, blood and tissue samples taken from fish were investigated by histopathological, electrophoretic and biochemical methods. As a result of the analyzes serum AST and ALT levels were decreased in the other groups compared to the negative control group and serum TAS levels were significantly increased in the 30 mg / L acrylamide group compared to the negative control group (P < 0.01). Compared with the negative control group, TOS levels were increased in all groups. When the electropherogram obtained from SDS-PAGE was examined, it was determined increases and decreases at 21 kD, 27 kD, 36 kD, 42 kD, 48 kD, 54 kD protein expressions in groups with different concentrations compared to the negative control group. It was observed that protein expressions were inhibited especially in the group treated with 20 mg / L acrylamide. As a result of histopathological examinations; increased degenerations were detected in the gill and liver tissues of fish due to the concentration of acrylamide. As a result; acrylamide treatment caused toxic effects on C. capoeta after this varying time intervals and concentrations.

Keywords:

Acrylamide Capoeta capoeta, fish, protein expression, AST, ALT, histopathology,

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