Determination of Cytotoxicity of Zinc 2-Bromobenzoate with Nicotinamide and N,N'-Diethylnicotinamide Complexes

Benzoic acid and its derivatives and their metal complexes, which have antimicrobial, anticancer, antituberculosis and antioxidant properties, are biologically active molecules. Although there are many studies on the biological activity of these compounds, studies on the determination of their toxicity are limited. In the presented study, the cytotoxic properties of the previously synthesized diaquabis(2-bromobenzoato-κO)bis(nicotinamide-κN1)zinc(II) (ZnBrBANA) and diaquabis(2-bromobenzoato-κO)bis(N,N'-diethylnicotinamide-κN1)zinc(II) (ZnBrBADENA) complexes were investigated. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a colorimetric method, was used to determine the cytotoxicity of complexes on human peripheral lymphocyte cells. Besides, cytotoxicity of dimethylsulfoxide (DMSO) which is crystal solvent and 2-bromobenzoic acid (BrBA), nicotinamide (NA), and N,N'-dietyhlnicotinamide (DENA) which are starting compounds of the complexes was also evaluated. According to the results of MTT method, It has been determined that both complexes and starting components except BrBA cause cytotoxicity on lymphocyte cells at the concentration range of 62.5-500 ppm. In addition, it was determined that the BrBA and DMSO at the same concentration range do not show any cytotoxic effect on lymphocyte cells. It was observed that the synthesized complexes were more toxic at each concentration than the starting components. Therefore, the toxic effects of the complexes used as drug active ingredients should be followed up with new studies.

Keywords:

Cytotoxicity, MTT assay, 2-Bromobenzoic acid Zinc Complex,

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