Maize is an important plant grown to obtain grain and silage, and is used in human and animal nutrition. In conventional maize breeding studies, stagnated line development studies are carried out for at least 7 years if a single generation is obtained in a year, while it is possible to develop 100% homozygous lines in a short period of 2 years with the in vivo maternal haploid technique. The in vivo maternal haploid technique is widely used in advanced maize breeding programs. The choice of donor or source material to be used for haploid induction depends on the purpose of the breeding program. Generally, breeders use F1 or F2 populations as source material for haploid induction. In this study; 30 F1 genotypes and their F2s were crossed with the inducer line. The putative haploid seed was identified by considering the R1-nj color marker, and the haploid induction rate was determined. The effect of the generations of the donor genotypes on the haploid induction ratio was compared by performing an independent sample test, and the haploid induction rate obtained from the F1 donors was found to be higher than the haploid induction rate of the F2 donors. It was determined that there was a change in the haploid induction rate as the genotypes changed within the F1 and F2 donor groups.