This study was carried out to determine the physical properties, biochemical and bioactive compounds of different apple cultivars grown in Yahyalı district of Kayseri province. The plant material of the research consisted of Scarlet Spur, Starking Delicious, Starkrimson Delicious, Golden Delicious, and Super Chief cultivars. In the study fruit weight, length, width, firmness, soluble solids content (SSC), titratable acidity, vitamin C, total phenolics, total flavonoids, and antioxidant activities (DPPH and FRAP assays) were investigated. In the findings, significant differences were determined between the fruit characteristics of the cultivars. The highest fruit weight (289.96 g) and length (32.61 mm) were measured from Scarlet Spur, while the highest fruit width (34.86 mm) was measured from Scarlet Spur and Starkrimson Delicious. SSC was determined between 11.65 (Super Chief)-14.30% (Starkrimson Delicious), and 0.47% (Super Chief)-1.13% (Golden Delicious) in cultivars for TA. Higher vitamin C content was measured in Starking Delicious cultivar (66 mg100 g-1) compared to others. In terms of total phenolic content, Scarlet Spur (38.2 g GAE kg-1) and Super Chief (36.5 g GAE kg-1) cultivars had the highest values, while Super Chief had the highest values in terms of total flavonoids (23.5 g QE kg-1), and antioxidant activity (FRAP, 490.7 mmol TE kg-1; DPPH, 1145.9 mmol TE kg-1). As a result, it was revealed the differences between the physical, biochemical, and bioactive contents of apple cultivars grown in Yahyalı district of Kayseri province.