Fatih Çağlar ÇELİKEZEN, Vedat TÜRKOĞLU, Mehmet FIRAT, Zehra BAŞ

Coriandrum sativum L. ve Chaerophyllum macropodum Boiss. (Apiaceae)’nin in vitro İnsan Plazma Anjiyotensin Dönüştürücü Enzim Üzerine Etkileri

Hipertansiyon dünya çapında çok önemli bir problemdir. Anjiyotensin dönüştürücü enzimin (ACE, EC.3.4.15.1.) inhibisyonu, hipertansiyon tedavisinin temeli olarak kabul edilir. Ancak sentetik ACE inhibitörlerinin bazı yan etkileri vardır. Bu nedenle, doğal ACE inhibitörleri geliştirmek için birçok çalışma yapılmaktadır. Bu sebeple, bu çalışma, Apiaceae'nin iki üyesi olan Coriandrum sativum ve Chaerophyllum macropodum'un insan plazma ACE'si üzerine potansiyel inhibitör etkilerini belirlemek için tasarlanmıştır. Bu amaçla, bitkilerin su ekstraktları kullanılmıştır. ACE inhibisyon aktivitesi spektrofotometrik olarak tespit edildi. Her iki bitki özütü de ACE aktivitesi üzerinde inhibe edici etki gösterdi. Elde edilen sonuçlar, Coriandrum sativum ve Chaerophyllum macropodum'un sırasıyla 0,7 mg/mL ve 1,14 mg/mL $IC_{50}$ değeriyle insan plazma ACE üzerine inhibe edici etkilere sahip olduğunu gösterdi. İnhibisyon tipini belirlemek için Lineweaver-Burk grafiği kullanıldı. İnhibisyon türleri geri dönüşümlü, yarışmasız inhibisyon olarak bulundu. Elde edilen sonuçlara göre, Coriandrum sativum ve Chaerophyllum macropodum’un ACE inhibisyon kapasitesine sahip, kan basıncını etkin bir şekilde dengelemek için kullanılabilecek, değerli fonksiyonel gıdalardır.

The Effects of Coriandrum sativum L. and Chaerophyllum macropodum Boiss. (Apiaceae) on human plasma angiotensin-converting enzyme (ACE) in vitro

Hypertension is a very important problem around the world. The inhibition of the Angiotensin-converting enzyme (ACE, EC.3.4.15.1.) is regarded as fundamental of hypertension treatment. However, synthetic ACE inhibitors have several side effects. For this reason, there are lots of studies to improve green ACE inhibitors. Therefore, this study was designed to determine the potential inhibitory effects of two members of Apiaceae, Coriandrum sativum and Chaerophyllum macropodum, on human plasma ACE. For this purpose, water extracts of the plants were used. ACE inhibition activity was detected spectrophotometrically. Both plant extracts showed an inhibitory effect on ACE activity. The obtained results showed that Coriandrum sativum and Chaerophyllum macropodum have inhibitory effects on human plasma ACE with an $IC_{50}$ value of 0.7 mg/mL and 1.14 mg/mL, respectively. Lineweaver-Burk graph was used to determine the inhibition type. The inhibition types were found as reversible noncompetitive. According to the obtained results, Coriandrum sativum and Chaerophyllum macropodum are valuable functional food with ACE inhibition capacity which may be used to balance blood pressure efficiently.

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