Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower

Farmakolojik şifalı bitkiler tarihteki ilk ilaçlarımızdır ve vücudumuza çay, baharat veya çiğ tüketerek farklı şekillerde alırız. Günümüzde daha etkili sentetik ilaçların yan etkileri nedeniyle farmakolojik bitkilerin kullanımına olan eğilim giderek artarak özellikle rutin hastalıkların tedavisinde ve bunlardan korunmada bitkilerden faydalanılmaktadır. Günlük yaşamımızda stres ve olumsuz çevresel koşullar, hücrede reaktif oksijen türlerinin konsantrasyonunu artırarak kanser ve Parkinson ve Alzheimer gibi çeşitli nörodejeneratif hastalıklarla ilişkili olduğu bilinen oksidatif strese neden olabilir. Antioksidanlar, reaktif oksijen türlerinin konsantrasyonunu düzenleyerek hücreyi hastalıklara karşı korur ve antioksidan olarak bilinen bitkisel fenolikler reaktif oksijen türlerinin süpürülmesi için güçlü adaylardır. Ayrıca bitki ve etken madde ile ilgili çalışmalar, Covid-19 salgını döneminde aşı geliştirme çalışmaları için önemli veriler sağladıkları için daha da önem kazanmıştır. Bu çalışma ile ileri araştırmalarda kullanılabilecek faydalı verilerin elde edilmesi amaçlanmıştır. Bu amaçla çeşitli farmakolojik özellikleri bilinen Asteraceae familyasının önemli üyelerinden biri olan Achillea arabica, Ağrı / Patnos'tan toplanmış ve herbaryum materyaline dönüştürülerek depolandı. Herbaryum bitkisinin çiçeklerinden DMSO ekstresi hazırlandı ve hazırlanan ekstrelerin, bazı in vitro antioksidan özellikleri, DNA koruyucu aktivitesi, ve HPLC ile 16 farklı fenolik içeriği kantitatif olarak belirlendi ve sonuçlar ilişkilendirilmeye çalışıldı.

Anahtar Kelimeler:

Achillea arabica, Antioksidan, DNA koruma, HPLC, Fenolik

Association of the phenolic content, DNA protective activity and some antioxidant properties in the Achillea arabica Kotschy Flower

Pharmacological herbs are our first medicines in history and we take them into our bodies in different ways by consuming tea, spices, or raw. Nowadays, due to the side effects of more effective synthetic medicines, the tendency to use pharmacological plants has increased and the benefits are provided from plants in the treatment of routine diseases and to protect from them. In our daily life, stress and adverse environmental conditions may cause oxidative stress by increasing the concentration of reactive oxygen species (ROS) in a cell and oxidative stress is known to be associated with cancer and various neurodegenerative diseases such as Parkinson’s and Alzheimer’s. Antioxidants protect the cell against diseases by regulating ROS concentration and herbal phenolics are known as antioxidants and strong candidates for this. In addition, studies on the herb and active ingredient have gained more importance during the Covid-19 epidemic period as they provide important data for vaccine development studies. This study was aimed to obtain useful data with this study that can be used in advanced research. For this purpose, Achillea arabica, which one of the important members of the Asteraceae family known whose various pharmacological properties, was collected from Ağrı/Patnos and stored by converting into herbarium material. DMSO extract was prepared from the flowers of the herbarium plant and some biological activities such as the determination of some in vitro antioxidant properties, DNA protective activity, and the phenolic content by HPLC were evaluated.

Keywords:

Achillea Arabica, Antioxidant, DNA protection, HPLC, Phenolic,

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