Whey Proteini İzolatının Rat Böbreğine Histolojik Etkileri

Amaç: Whey proteini diğer yapısal proteinlere göre daha fazla miktarda dallı zincirli aminoasit içeren tam bir proteindir.Bu proteinin uzun süreli kullanımının böbrek dokusunda meydana getirdiği histolojik değişiklikler henüz araştırılmamıştır.Bu çalışmada düzenli olarak bir ay boyunca gavaj ile whey protein izolatı verilen ratların böbrek dokusundaki histolojikparametrelerin değerlendirilmesi amaçlanmıştır.Gereç ve Yöntem: Bu çalışma Afyonkarahisar Sağlık Bilimleri Üniversitesi Tıp Fakültesi Fizyoloji Anabilim Dalı ve Histoloji veEmbriyoloji Anabilim Dalı’nda, 250-300 g ağırlığında 30 adet Wistar Albino erkek sıçan kullanılarak yapılmıştır. Her grupta10’ar sıçan olacak şekilde kontrol, sham ve whey proteini uygulanan grup olarak 3 gruba ayrılmıştır. Kontrol grubunaherhangi bir uygulama yapılmazken, sham grubuna 1 mL çeşme suyu, whey proteini grubuna 6 g/kg/gün whey proteini 1 mLçeşme suyunda çözünerek bir ay boyunca gavajla verilmiştir. Böbrek dokusundaki genel morfolojik değişiklikleri incelemekiçin Hematoksilen-Eozin, PAS ve Masson-Trikrom boyamaları yapılmıştır. Böbreklerdeki IL-6, FGF-1, p53 ekspresyonlarınıdeğerlendirmek için dokular immunohistokimyasal olarak boyanmıştır.Bulgular: Whey protein grubunda hafif mezengial matriks genişlemeleri görülmekle beraber hem bu grupta hem de diğergruplarda bunun dışında başka bir patolojiye rastlanmamıştır. Whey protein grubunda IL-6 ve FGF-1 boyanmaları diğergruplara göre istatistiksel olarak anlamlı bir artış göstermiştir (p

Histological Effects of Whey Protein Isolate on Rat Kidney

Aim: Whey protein is a complete protein that contains more amount of branched chain amino acids than other structural proteins. Histological changes in long-term use of this protein in kidney tissue have not been studied yet. In this study, it was aimed to evaluate the histological parameters in the kidney tissue of rats given whey protein isolate regularly for a month. Material and Method: This study was carried out using 30 Wistar Albino male rats weighing 250-300 g in Afyonkarahisar Health Sciences University Faculty of Medicine, Physiology Department and Histology and Embryology Department. Control, sham and whey protein groups were divided into 3 groups with 10 rats in each group. While no application was made to the control group, 1 mL of tap water was given to sham group and 6 g/kg/day of whey protein was dissolved in 1 mL of tap water then given to whey protein group for 1 month. Hematoxylin-Eosin, PAS and Masson-Trichrome staining were performed to examine general morphological changes in kidney tissue. Tissues were immunohistochemically stained to evaluate IL-6, FGF-1, p53 expressions in kidneys. Results: Although mild mesengial matrix dilatations were seen in the whey protein group, no other pathology was observed in this group and other groups. IL-6 and FGF-1 dyes in the whey protein group showed a statistically significant increase compared to other groups (p

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