Maternal Diyetle Fruktoz Alımının Anne ve Yavru Sıçanlarda Trigliserit ve Serbest Yağ Asidi Düzeyleri Üzerine Etkisi

Amaç: Bu çalışmanın amacı gebelik öncesi, gebelik ve laktasyon dönemlerinde maternal diyetle fazla miktarda fruktoz alımının anne ve yavru sıçanlarda kanda bazı lipit parametreleri ile karaciğerde trigliserit birikimi üzerine etkilerini incelemektir. Gereç ve Yöntem: Çalışmaya 10 adet aynı tür (Sprague Dawley) ve soydan gelen anne sıçan (her grupta n=5 adet), 23 adet fruktoz ve 29 adet maltodekstrin grubundan doğan toplam 52 adet yavru sıçan ile eşleşme için 2 adet erkek sıçan dahil edilmiştir. İki haftalık arınma (wash-out) süresi sonunda birinci gruba %20 w/v konsantrasyonunda fruktoz, ikinci gruba da aynı miktarda maltodekstrin verilmiştir. On iki haftalık diyet müdahalesi sonrası gebe kalan sıçanlar gebelik ve laktasyon dönemlerinde aynı şekilde beslenmiş ve laktasyon döneminin sonunda organ ve doku izolasyonu için ötanazi yapılarak deney sonlandırılmıştır. Sıçanların su ve yem tüketimleri kaydedilmiş, kanda trigliserit ve serbest yağ asitleri ile karaciğerde trigliserit birikimleri uygun olan kolorimetrik veya florometrik yöntemle analiz edilmiştir. Bulgular: Çalışma sonucunda sıçanların ortalama yem ve su tüketimleri ile terminal dönem vücut ağırlıkları için gruplar arasında istatistiksel olarak anlamlı fark bulunmamıştır. Yüksek miktarda fruktoz alan anne sıçanlar ile maternal diyetle fruktoza maruz kalan yavru sıçanlarda plazma ve karaciğer trigliserit miktarları ile plazma toplam serbest yağ asitleri düzeyinde artış saptanmıştır (p=0.05). Sonuç: Sonuç olarak bu çalışma, fruktoza uzun dönem maruziyetin gelişimsel programlama hipotezine göre trigliserit ve yağ asit metabolizmasını etkileyerek erişkin başlangıçlı kardiyovasküler hastalıkların oluşum riskini artırabileceğini göstermiştir.

The Effect of Maternal Fructose Intake in Terms of Triglyceride and Free Fatty Acid Levels in Mother Rats and Pups

Aim: The aim of this study was to examine the effects of high fructose intake during pre-conception, throughout pregnancy and lactation on some lipid parameters in the plasma and triglyceride accumulation in the liver of both mother rats and pups. Materials and Methods: This study was carried out on ten mother rats (n=5 for each group) total 52 pups (23 pups in the fructose group, 29 pups in the maltodextrin group) and two male rats for mating which were inbred Sprague Dawley strain. After two-weeks of wash out period rats were divided into two groups. Fructose (20% w/v) in water was administered to the first group and the same amount of maltodextrin was administered to the second group. After 12-weeks of dietary manipulation period the rats were mated for pregnancy. Rats were kept on same diets during pregnancy and lactation periods. At the end of lactation period, tissues and organs were isolated, then the animals were euthanised. Feed and water intake were recorded during the study. Triglyceride and free fatty acids in the blood and triglyceride accumulation in the liver were analysed with colorimetric or flourimetric methods as required. Results: As a result, there were no significant differences on feed and water intake, and terminal body weights of the rats. Furthermore, plasma and liver triglyceride levels, plasma free fatty acids of mothers and pups with high fructose exposure were found to be elevated (p=0.059). Conclusion: In conclusion, this study showed that chronic high fructose intake may increase the risk of cardiovascular diseases in the later life of offspring by affecting triglyceride and fatty acid metabolism via developmental programming.

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