Farklı çapraz bağlayıcılar ile hazırlanmış poliakrilamid hidrojellerinin değerlendirilmesi

Çapraz bağlı poliakrilamid (PAAm) hidrojelleri; fiziksel özellikleri üzerine çapraz bağlayıcı türü etkisinin incelenmesi amacıyla sentezlenmiştir. Bu amaçla; N,N’-metilenbisakrilamid (MBA), trietilen glikol dimetakrilat (TEGDMA), glutaraldehit (GLU), divinilsülfon (DVS), N,N’-Diallil L-ltartardiamit (DATD) ve epiklorhidrin (ECH;) çapraz bağlayıcılar olarak seçilmiş ve bu hidrojeller sırasıyla PAAm-MBA, PAAm-TEGDMA, PAAm-GLU, PAAm-DVS, PAAm-DATD, PAAm-ECH olarak isimlendirilmiştir. Tüm çapraz bağlayıcılar için nçapraz bağlayıcı/nmonomer oranı=%2.5 olarak sabitlenmiştir. Amonyumpersülfat (APS) ve N,N,N’,N’-tetrametiletilendiamin (TEMED); redoks başlatıcısı-hızlandırıcısı olarak seçilmiş ve PAAm hidrojelleri; çapraz bağlayıcıların bulunduğu ortamda monomerlerin radikalik polimerleşmesi ile hazırlanmıştır. PAAm hidrojellerinin şişme ve difüzyon parametrelerinin hesaplanabilmesi amacıyla kinetik şişme çalışmaları; deiyonize suda (DS) ve 25 oC’de gerçekleştirilmiştir. Deneysel şişme verileri; şişme prosesinin 2o’den kinetiğe uyduğunu göstermiştir. PAAm hidrojellerinin şişme dereceleri; PAAm-ECH>PAAm-DVS>PAAm-MBA=PAAm-DATD>PAAm-GLU>PAAm-TEGDMA olarak bulunmuştur. PAAm hidrojelleri; su moleküllerinin hidrojellere difüzyon türünün Fick tipi olmayan difüzyon türü olduğunu gösteren ve değeri 0.57 ve 0.80 aralığında değişen sayılara (n) sahiptir. PAAm hidrojellerinin çapraz bağlar arası ortalama mol kütlesi ( ), çapraz bağ yoğunluğu (q) ve gözeneklilik (P) gibi ağ yapı parametreleri hesaplanmıştır. Denge şişme çalışmaları; protein (sığır serumu albumini, BSA), bakteriyel polisakkarit (dekstran, DEX), vitamin (nikotinamid, NAD) ve ilaç etken maddesi (8-hidroksi 7-iyodokinolin-5-sülfonik asit, HKSA) çözeltilerinde de yapılmıştır. Şişme ve ağ yapı parametreleri, PAAm hidrojelleri; yapısı protein, karbohidrat, vitamin ve ilaç etken moleküllerine benzeyen moleküller ile etkileştirildiğinde şişme ve adsorpsiyon özelliklerinin tahmin edilmesinde yol gösterici olacaktır.

Evaluation of polyacrylamide hydrogels clamped with different crosslinkers

Crosslinked polyacrylamide (PAAm) hydrogels were synthesized to investigate the effect of crosslinker type onto pysical properties of PAAM gels. With this aim; N,N’-methylenebis acrylamide (MBA), triethylene glycol dimethacrylate (TEGDMA), glutaraldehyde (GLU), divinyl sulfone (DVS), N,N′-diallyl L-tartardiamide (DATD), and epichlorohydrin (ECH) were selected as crosslinkers and these hydrogels were called as PAAm-MBA, PAAm-TEGDMA, PAAm-GLU, PAAm-DVS, PAAm-DATD, and PAAm-ECH, respectively. The ratio of ncrosslinker/nmonomer was fixed as 2.5% for all crosslinkers. PAAMs were prepared by radical polymerization of monomers in presence of crosslinkers and redox initiator-accelerator, i.e., ammonium persulphate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED). Kinetic swelling studies of hydrogels were carried out in deionize water (DW) at 25 ◦C for calculating swelling and diffusion parameters of the PAAms. Experimental data of swelling suggest clearly that the swelling processes obey second-order kinetics. Swelling degrees of the PAAms were found as PAAm-ECH>PAAm-DVS>PAAm-MBA=PAAm-DATD>PAAm-GLU>PAAm-TEGDMA. PAAms has numbers (n) between 0.57 and 0.80 indicating type of diffusion of water molecules to the hydrogels is non-Fickian type. Network parameters of PAAMs such as average molecular weight between crosslinks ( ), crosslink density (q), and porosity (P) were calculated. Equilibrium swelling studies were also realized in solutions of protein (bovine serum albumine, BSA), bacterial polysaccharide (dextran, DEX), vitamine (nicotineamide, NAD), and drug (8-hydroxy-7-iodoquinoline-5-sulfonic acid, HKSA). Swelling and network parameters of the PAAms will be guide to estimate of swelling and adsorption behaviours of PAAm hydrogels clamped with different crosslinkers when they are interacted with molecules such as protein, polysaccharide, vitamine, and drug molecules.

Keywords:

Polyacrylamide, crosslinker, hydrogel swelling degree, porosity,

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