Zozan GARİP, Füsun TEMAMOĞULLARI, Pinar AKSU KILIÇLE

Ratlarda Nikel Sülfatın Genotoksisitesine Karşı Likopenin Koruyucu Etkisi

Amaç: Endüstrilerde geniş kullanım yeri olan nikelin teratojenik, kanserojenik, immünotoksik ve genotoksik etkileri vardır. Bu çalışmada, nikel sülfatın sebep olduğu genotoksisiteye karşı antioksidan özelliğe sahip likopenin koruyucu etkileri araştırılmıştır. Gereç ve Yöntem: Çalışmada toplam 24 adet rat (Wistar albino) dört gruba ayrılmıştır. Grup I (kontrol) günlük olarak serum fizyolojik intraperitonal (i.p.) ve mısır yağı oral gavajla (0.5 ml); Grup II nikel sülfat (20 mg/kg, i.p.) serum fizyolojik içerisinde çözdürülerek; Grup III likopen mısır yağı (0.5 ml) süspansiyon haline getirilerek 20 mg/kg dozlarında oral gavajla; Grup IV likopen mısır yağı (0.5 ml) içerisinde süspansiyon haline getirilerek 20 mg/kg dozlarında oral gavajla verildikten 2 saat sonra nikel sülfat (20 mg/kg, i.p.) 21 gün boyunca uygulanmıştır. Genotoksisite testlerinden biri olan kemik iliğinden mikronükleus testiyle mikronükleuslu polikromatik eritrositler (MNPCE) ve polikromatik eritrositler (PCE) incelenmiştir. Bulgular: Koruyucu amaçlı olarak likopen uygulamasının MNPCE düzeylerini nikel sülfat grubuna göre anlamlı olarak (p

Anahtar Kelimeler:

Genotoksisite, Likopen, Nikel Sülfat, Rat

Protective Effect of Lycopene Against Genotoxicity of Nickel Sulphate in Rats

Aim: Nickel, which has a wide usage area in industries, has teratogenic, carcinogenic, immunotoxic, and genotoxic effects. In this study, the protective effects of lycopene, which has antioxidant properties, were investigated against genotoxicity caused by nickel sulphate. Materials and Methods: In the study, a total of 24 rats (Wistar albino) were divided into four groups: Group I (control) was given daily saline intraperitoneally (i.p.) and corn oil orally by gavage (0.5 ml); Group II was given nickel sulphate (20 mg/kg, i.p.) dissolved in physiological saline; Group III was given lycopene suspended in corn oil (0.5 ml) by oral gavage at 20 mg/kg doses; Group IV was given both lycopene suspended in corn oil (0.5 ml) by oral gavage at 20 mg/kg doses, and 2 hours later, nickel sulphate (20 mg/kg, i.p.) for 21 days. Micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes (PCE) were examined by bone marrow micronucleus test, which is one of the genotoxicity tests. Results: It was determined that the administration of lycopene for preventive purposes decreased the MNPCE levels significantly (p

Keywords:

Genotoxicity, Lycopene, Nickel Sulphate, Rat,

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