Bilal Cem LİMAN, Narin LİMAN, Yasin TEKELİ, Ergül ERGEN, Ural Kemal KAVRAAL

Propil Tiyourasil (PTU) ve L-Tiroksin ile Oluşturulan Deneysel Hipo- ve Hipertiroidizm Erişkin Sıçanların Hipokampüsünde Doku Lipid Peroksidasyonu, Glutatiyon ve Antioksidan Enzim Düzeyleri Üzerine Araştırmalar

Amaç: Bu çalışmanın amacı deneysel hipo- ve hipertiroidizm oluşturulan sıçan modelinde hipokampüsde lipid peroksidasyon ve düzeyleri ile antioksidan enzim aktivitelerinin değişip değişmediğini ortaya koymaktır. Materyal ve Metod: Çalışmada kullanılan 72 yetişkin erkek Wistar albino sıçan aşağıdaki şekilde gruplandırıldı: (1) kontrol grubu (2) hipotiroidizm grubu: 4 hafta boyunca 10 mg/kg/gün propiltiourasil (PTU)’in intraperitoneal enjeksiyonu ile indüklendi; (3) hipertiroidizm grubu: 4 haftalık tiroksin enjeksiyonu (0.3 mg/kg/gün) ile indüklendi. Hipo ve hipertirodi oluşturulan sıçanların hipokampüslerinde lipid peroksidasyon düzeyleri, glutatyon (GSH) düzeyi, antioksidan enzimlerden süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx) ve glutatyon redüktaz (GR) düzeyleri ELISA ile incelendi. Bulgular: Çalışmamızın ELISA analiz sonuçları hipo- ve hipertiroidizmin hipokampal lipid peroksidasyonunu etkilemediğini, ancak hipotiroidi oluşturulan sıçanların hipokampüs örneklerinde SOD düzeylerinin kontrol grubuna göre anlamlı olarak düşük olduğunu (P

Anahtar Kelimeler:

Hipotiroidizm, Hipertiroidizm, propiltiyourasil, L-tiroksin, Hipokampüs, Antioksidan enzim sistemler

Investigations on Tissue Lipid Peroxidation, Glutathione and Antioxidant Enzyme Levels in the Hippocampus of Adult Rats in Experimental Hypo- and Hyperthyroidism Induced by Propyl Thiouracil (PTU) and L-Thyoxine

Objective: This study aimed to explore the effect of experimental hypo- and hyperthyroidism on the lipid peroxidation and glutathione levels, and the activities of antioxidant system in the rat hippocampus. Material and Methods: The study included 72 adult male Wistar albino rats which were grouped as follows: (1) control group (2) hypothyroidism group: induced by intraperitoneal injection of 10 mg/kg/day propylthiouracil (PTU) for 4 weeks; (3) hyperthyroidism group: induced by 4-week thyroxine injection (0.3 mg/kg/day). The levels of lipid peroxidation, glutathione (GSH), antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) in the hippocampus of rats with hypo- and hyperthyroidism were examined using ELISA techniques. Conclusion: It can be said that TH disorder may affect cognitive performance by causing some changes in the antioxidant defense system of the hippocampus.

Keywords:

Hypothyroidism, Hyperthyroidism, propylthiouracyl, L-thyroxine, Hippocampus, Antioxidant enzyme systems,

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