Mine GÜLTEKİN ÖZGÜVEN, Beraat ÖZÇELİK, Kadriye Nur KASAPOĞLU, Ceren GÜNGÖR, Esra Nur ERTÜRK

İnce Film Hidrasyon ve Mikrofluidizasyon Yöntemleri KullanılarakPolifenollerin Yüksek Verimle Nanokapsüllere Yüklenmesi

Bu çalışmada, fenolikçe zengin siyah kuşburnu meyvesi ekstraktı ince film hidrasyon ve mikrofluizidasyon yöntemleri birlikte kullanılarak nano boyuttaki lipozomlara yüksek verimle yüklenmiştir. Hazırlanan nanolipozomların stabilitesinin arttırılması amacıylanegatif elektrik yüklü olan nanolipozomlar elektrostatik depozisyon tekniği ile pozitif yüklü bir biyopolimer olan kitosan ilekaplanmıştır. Bunun için farklı konsantrasyonlarda (%0.1, %0.2, %0.3, %0.4 ve %0.5) çalışılarak optimum kaplama için gereklikitosan konsantrasyonu araştırılmıştır. Bu amaçla, hazırlanan nanolipozom kapsülleri ortalama partikül boyutu ve zeta potansiyeli ölçümleri ile karakterize edilmiştir. Enkapsülasyon veriminin belirlenmesi için toplam fenolik madde içerikleri (TPC) -Folin Ciocalteuyöntemi ve antioksidan kapasiteleri (CUPRAC ve DPPH yöntemleri) analizleri gerçekleştirilmiştir. Çalışmada kullanılan %0.1 kitosankonsantrasyonu hariç (çökme gözlendi) diğer kitosan konsantrasyonlarında (%0.2, %0.3, %0.4 ve %0.5) kaplama başarılı olmuştur.En yüksek enkapsülasyon verimine %0.3 kitosan konsantrasyonu ile kaplanan nanolipozomlarda ulaşılmıştır. Buna göre,enkapsülasyon verimi TPC’ye göre %92, CUPRAC’a göre %87, DPPH’e göre %83 olarak belirlenmiştir. Sonuç olarak, ince filmhidrasyon ve mikrofluizidasyon yöntemlerinin birlikte kullanılmasıyla yüksek enkapsülasyon verimli nanolipozom üretimininmümkün olduğu anlaşılmıştır.

Loading Polyphenols into Nanocapsules with High Encapsulation Efficiency Using Thin Film Hydration and Microfluidization

In this study, phenolic-rich black rosehip fruit extract was loaded into nano-sized liposomes with high efficiency by using thin-filmhydration and microfluidization methods together. In order to increase the stability of the prepared nanoliposomes, negatively chargednanoliposomes were coated with chitosan, a positively charged biopolymer, by electrostatic deposition technique. For this, thechitosan concentration required for optimum coating was investigated by working at different concentrations (0.1%, 0.2%, 0.3%,0.4% and 0.5%). For this purpose, the prepared nanoliposome capsules were characterized by mean particle size and zeta potentialmeasurements. To determine the encapsulation efficiency, total phenolic content (TPC) by Folin Ciocalteu method and antioxidantcapacity (CUPRAC and DPPH methods) analyzes were performed. Coating was successful at other chitosan concentrations (0.2%,0.3%, 0.4% and 0.5%), except for the 0.1% chitosan concentration (aggregation was observed). The highest encapsulation efficiencywas achieved in nanoliposomes coated with 0.3% chitosan concentration. Accordingly, the encapsulation efficiency was determinedas 92%, 87% and 83% in terms of TPC, CUPRAC and DPPH assays, respectively. In conclusion, it has been understood that it ispossible to produce nanoliposomes with high encapsulation efficiency by combining the thin film hydration and microfluidizationmethods together.

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