Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family
Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family
Lamiaceae family is one of the members of important plants, using as a agents for alternative medicine treatments. Our aim was toinvestigate both antigenotoxic and genotoxic effects three different plants species belonging to Lamiaceae family: Stachys annua,Scutellaria salviifolia, Nepeta nuda in Amasya, using SMART (Drosophila melanogaster somatic mutation and recombination test)and also plasmid DNA interaction method, respectively. SMART analyses revealed that genotoxic effect of ethyl methanesulfonate(EMS) was eliminated by plant extract of S. salviifolia, indicating antigenotoxic effects. Different extract concentrations [(0.1, 0.25,0.50 and 1 % (v/v)] of other plants species decrased the flecks number on D. melanogaster but the results did not show any statisticalsignificance (p>0.05). In addition to SMART, interaction with pUC18 plasmid DNA was also analysed by using all concentrations(50 µg/mL-3.125 µg/mL) of plant extracts N. nuda and S. annua. The extracts did not cause changed in Form I DNA (supercoil) buttrasferred from Form II DNA (open circular) to Form III DNA. Moreover, there was no effect of S. salviifolia plant extracts on DNAand band density. SMART results were supported by DNA interaction analyses, cocncluding that S. salviifolia plants indicated thehigh-impact antigenotoxic effect, but not genotoxic effects.
___
- Amkiss, S., Dallouh, A., Idaomar, M., Amkiss, B. 2013.
Genotoxicity and anti-genotoxicity of fennel plant
(Foeniculum vulgare Mill) fruit extracts using the somatic
mutation and recombination test (SMART). African Journal
of Food Science 7(8), 193-197.
- Asmafiliz, N., Kılıç, Z., Öztürk, A., Süzen, Y., Hökelek, T.,
Açık, L., Çelik, Z.B., Koç, L. Y., Yola, M.L., Üstündağ, Z.
2013. Phosphorus-Nitrogen Compounds: Part 25.
Syntheses, Spectroscopic, Structural and Electrochemical
Investigations, Antimicrobial Activities, and DNA
Interactions of Ferrocenyl di amino cyclotri phosphazenes.
Journal Phosphorus, Sulfur, and Silicon and the Related
Elements 188, 1723-1742.
- Avalos, A., Haza, A.I., Drosopoulou, E., Mavragani-Tsipidou,
P., Morales, P. 2015. In vivo genotoxicity assesment of
silver nanoparticles of different sizes by the Somatic
Mutation and Recombination Test (SMART) on
Drosophila. Food and Chemical Toxicology 85, 114-119.
- Ayar, A., Uysal, H. 2013. Genotoxic and safety assessment of 2
parabens in somatic cells of in vivo Drosophila
melanogaster. Turk Journal of Biology 37, 683-688.
- Baser, K.H.C. 1993. Essential Oils of Anatolian Lamiaceae: A
Profile. Acta Horticulturae 333, 217-238.
- Baytop, T. 1999. Therapy with Plants in Turkey; Past and
Present. Nobel Tıp Press, (2nd ed), Istanbul (in Turkish).
Bhattacharya, S. 2011. Natural antimutagens: a review.
Research Journal of Medical Plant 5, 116-126.
- Boubaker, J., Skandrani, I., Bouhlel, I. 2010. Mutagenic,
antimutagenic and antioxidant potency of leaf extracts from
Nitraria retusa. Food and Chemical Toxicology 48, 2283-
2290.
- Chung, P.Y., Chung, L.Y., Ngeow, Y.F., Goh, S.H., Imiyabir,
Z. 2004. Antimicrobial activities of Malaysian plant
species. Pharmaceutical Biology 42, 292–300.
- De Mattos, J.C.P., Dantas, F.J.S., Bezerra, R.J.A.C., BernardoFilho,
M., Cabral-Neto, J.B., Lage, C.A.S., Leitao, A.C.,
Caldeira-de-Araujo, A. 2000. Damage induced by stannous
chloride in plasmid DNA. Toxcollogy Letter 116, 159-163.
- Donya, S.M., Nancy, H.I. 2012. Antimutagenic potential of
Cynara scolymus, Cupressus sempervirens and Eugenia
jambolana against paracetamol-induced liver cytotoxicity.
Journal of American Science 8, 61-67.
- Essawi, T., Srour, M. 2000. Screening of some Palestinian
medicinal plants for antibacterial activity. Journal of
Ethnopharmacology 70, 343-349.
- Enjun, G., Lei, L., Mingchang, Z., Qiong, W. 2009. Sysnthesis,
characterization and Biological Activity an İntramolecular
Stacking Zinc (II) Complex. Chininese Journal of Chemical
Engineering 27, 1285-1290.
- Frei, H., Clements, J., Howe, D. 1992. The genotoxicity of
anticancer drug mitoxantrone in somatic and germ cells.
Mutation Research 279, 21-33.
- Graf, U., Würgler, F.E, Katz, A.J. 1984. Somatic mutation and
recombination test in Drosophila melanogaster.
Environmental and Molecular Mutagenesis 6, 153-188.
- Kianbakht, S., Jahaniani, F. 2003. Evaluation of antibacterial
activity of Tribulus terrestris L. growing in Iran. Iranian
Journal of Pharmacology and Therapeutics 2, 22-24.
- Kumar, A., Kaur, R., Arora, S. 2010. Free radical scavenging
potential of some Indian medicinal plants. Journal Medical
Plants Research 4, 2034-2042.
- Kunduhoglu, B., Pilatin, S., Caliskan, F. 2011. Antimicrobial
screening of some medicinal plants collected from
Eskisehir. Turkey Fresenius Environmental Bulletin 20(4),
945-952.
- Lee, J., Kim, H., Jang, Y. 2002. Antoxidant property of an
ethanol extract of the stem of Opuntia ficus-indica var.
Saboten Journal Agriculture Food Chemistry 50, 6490-
6496.
- Legin, A.A., Jakupec, M.A., Bokach, N.A., Tyan, M.R.,
Kukushkin, V.Y., Keppler, B.K. 2014. Guanidine platinum
(II) complexes: synthesis, in vitro antitumor activity, and
DNA interactions. Journal Inorganik Biochemical 133, 33-
39.
- Mitchell, R.E., Teh, K.L. 2005. Antibacterial iminopyrrolidines
from Burkholderia plantarii, a bacterial pathogen of rice.
Organic & Biomolecular Chemistry 3, 3540-3543.
- Mutlu, G., Elmas, G., Kılıç, Z., Hokelek, T., Koç, L.Y., Türk,
M., Açık, L., Aydın, B., Dal H. 2015. Phosphorus–nitrogen
compounds: Part 31. Syntheses, structural and stereogenic
properties, in vitro cytotoxic and antimicrobial activities,
and DNA interactions of bicyclo tetra phosphazenes
containing bulky side group. Inorganica Chimica Acta 436,
69–81.
- Nemeth, E., Bernath J. 2008. Biological activities of yarrow
species (Achillea spp). Current Pharmaceutical Design 14,
3151-3167.
- Ong, C., Yung, L.L., Cai, Y., Bay, B., Baeg, G. 2014.
Drosophila melanogaster as a model organism to study
nanotoxicity. Nanotoxicology 9(3), 396-403.
- Orsolin, P.C., Silva-Oliveira, R.G., Nepomuceno, J.C. 2015.
Modulating effect of synthetic statins against damage
induced by doxorubicin in somatic cells of Drosophila
melanogaster. Food and Chemical Toxicology 81, 111-119.
- Salem, M.Z.M, Ali, H.M., El-Shanhorey, N.A., Ahmed, A.M.
2013. Evaluation of extracts and essential oil from
Callistemon viminalis leaves: Antibacterial and antioxidant
activities, total phenolic and flavonoid contents. Asian
Pacific Journal of Tropical 6, 785-791.
- Sarac, N., Ugur, A. 2007. Antimicrobial activities and usage in
folkloric medicine of some Lamiaceae species growing in
Mugla. Turkey EurAsian Journal of BioSciences (EurAsia
J BioSci) 4, 28-37.
- Si, X.T., Zhang, M.L., Shi, Q.W., Kiyota, H. 2006. Chemical
constituents of the plants in the genus Achillea. Chemical
Biodiversity 3, 1163-1180.
- Skandrani, I., Ben Sghaier, M., Neffati, A. 2007. Antigenotoxic
and free radical scavenging activities of extracts from
Moricandia arvensis. Drug and Chemical Toxicology 30,
361-382.
- Soares, B.O., Oliveira, M.B.N., Mansur, E., Dantas, F.J.S., De
Mattos, J.C.P., Caldeira-de-Araujo, A., Gagliardi, R.F.
2014. Effect of extracts from field and in vitro plants of
Petiveria alliacea L. On plasmidial DNA. Journal of
Medicinal Plant Research 8(35), 1101-1109.
- Unal, E.L., Mavi, A., Kara, A.A., Cakir, A., Şengül, M.,
Yildirim, A. 2008. Antimicrobial and antioxidant activities
of some plants used as remedies in Turkish traditional
medicine. Pharmaceutical Biology 46, 207-224.
- Uysal, H., Kızılet, H., Ayar, A., Taheri, A. 2015. The use of
endemic Iranian plant, Echium amoenum, against the ethyl
methanesulfonate and the recovery of mutagenic effects.
Toxicology and Industrial Health 31(1), 44-51.