Optimization of wheat (Triticum aestivum L.) anther culture-derivad embryos transformation

Partikül bombardıman yöntemi, buğday anter kültürü kaynaklı embriyolara gen aktarım metodu ile geliştirilmeye çalışılmıştır. Bu araştırmada, "Pavon 76" çeşiti anter kültürü kaynaklı embriyoların eldesi için kullanılmıştır. Mikroprojektil bombardımanı ile aktarılan Gus geninin geçici ifadesi anter kültürü kaynaklı embriyolarda tespit edilmiştir. Bombardımandan önce, Ne ozmotik ortam ve embriyoların Liang's 85D12 tuz ve organikleri ile yıkanması Gus geninin geçici ifadesini önemli derecede artırmıştır.

Partikül bombardımanı ile buğday (Triticum aestivum L.) anter kültürü kaynaklı embriyolara gen aktarımının optimizasyonu

Wheat transformation method is being developed using microprojectile bombardment of anther culture derived embryos. Anther culture derived embryos were initiated from the spring cultivar 'Pavon 76'. Transient expression of the gus gene was detected in which anther culture derived embryos were treated via microprojectile bombardment. N6 osmatic medium and washing embryos with Liang's 85D12 salt and organics prior to bombardment increased the transient expression of the gus gene.

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