Harun ALP, Metin OGÜN, İsmail AYTEKİN, Onur ATAKİŞİ

Ratlarda akut malathion toksisitesinin neden olduğu oksidatif stres üzerine kafeik asit fenetil ester ve elajik asit’in etkileri

Araştırma akut malathion (MAL) toksikasyonuna maruz kalan ratların akciğer, karaciğer ve böbrek dokularında oluşan malondialdehit (MDA), redükte glutatyon (GSH) ve nitrik oksit (NO) aktiviteleri üzerine kafeik asit fenetil ester (CAPE) ve elajik asitin (EA) etkilerini incelemek amacıyla yapıldı. Toplam 36 adet Sprague Dawley soyunda yetişkin dişi rat, randomize şekilde her grupta 6 adet olmak üzere 6 gruba (kontrol, CAPE, EA, MAL, MAL+CAPE, MAL+EA) ayrıldı. Akciğer, karaciğer ve böbrek dokularında redükte GSH, MDA ve NO düzeyleri spektrofotometrik yöntemle kolorometrik olarak ölçüldü. Kontrol grubu ile CAPE ve EA gruplarını redükte GSH, MDA ve NO aktiviteleri açısından karşılaştırdığımızda, gruplar arasında istatistiksel olarak önemli bir fark saptanmadı. MAL’un karaciğer dokusunda MDA düzeylerini önemli derecede arttırdığı, ancak CAPE’nin MAL’un toksik etkisini engelleyerek MDA seviyesini anlamlı şekilde azalttığı belirlendi. Ayrıca, MAL’un akciğer, karaciğer ve böbrek dokularında redükte GSH düzeylerini azalttığı, buna karşın CAPE ve EA’in ise redükte GSH düzeylerini arttırdığı belirlendi. MAL’un neden olduğu şiddetli doku hasarına bağlı olarak NO düzeylerinin de önemli derecede arttığı, fakat CAPE ve EA’in NO düzeylerini azalttığı tesbit edildi (p

The effects of caffeic acid phenethyl ester and ellagic acid on oxidative stress created by acute malathion toxicity in rat

The aim of this study was to investigate the effects of caffeic acid phenethyl ester (CAPE) and ellagic acid (EA) on acitivities of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) in rat lung, liver and kidney tissues in acute malathion (MAL) toxicity. A total of 36 mature female Sprague Dawley rats were randomly divided into 6 groups of 6 including control, CAPE, EA, MAL, MAL+CAPE, MAL+EA. Reduced GSH, MDA and NO levels in lung, liver and kidney tissues were measured colormetrically by spectrophotometer. When the unmedicated control group was compared with the CAPE and EA groups, there was no statistically significant difference between the groups in terms of GSH, MDA and NO acitivities. It was determined that MDA levels in liver tissue were significantly increased by MAL, but CAPE significantly reduced the MDA levels by blocking the toxic effect of MAL. Also, it was observed that the reduced GSH levels in the liver tissues of the groups given MAL were reduced, but CAPE and EA increased reduced GSH levels. The NO levels of the groups given MAL were significantly increased dependent on the severe tissue damage created by MAL, but CAPE and EA reduced those levels. As a result, it was concluded that CAPE and EA, which showed similar effects to each other, could be used for protection against the severe oxidative stress and damage tissue caused by acute MAL poisining.

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