Ekrem KİREÇCİ, Hakan USLU, Mustafa SAVAŞÇI

Kars ve Sarıkamış çevresindeki içme suyu kaynaklarından membran filtrasyon yöntemi ile Escherichia coli izolasyonu

Bu çalışmada, Kars ve Sarıkamış askeri birliklerinde kullanılan içme sularının mikrobiyolojik kalitesi ve Escherichia coli’nin varlığı araştırıldı. Bu araştırmada, fekal indikatörler membran filtrasyon yöntemi ile izole edildi. Bu yöntem, sulardan fekal koliformların belirlenmesi için standart yöntemlere alternatif olarak kabul edilmektedir.Bu amaçla, su kaynaklarından 6 ay süresince toplanan 1469 adet su numunesi çalışmaya alındı. Numuneler, 0.45μm por aralığına sahip sellüloz membran filtrelerden süzüldükten sonra her bir filtre Endo-NKS besiyerlerine yerleştirildi. Daha sonra her besiyeri 37 ºC’de 24 saat inkübe edildi. Besiyerlerinde üreyen kırmızı renkli koloniler seçilerek biyokimyasal yöntemlerle E.coli identifikasyonu yapılmıştır. Sonuçta, 1469 adet su numunesinin %30’unda E.coli izole edildi. İçme sularındaki E.coli varlığı fekal bir kontaminasyonu gösterdiğinden, bu durumun su kaynaklı infeksiyonlara yol açabileceği düşünülmektedir.

Isolation of Escherichia coli from drinking water in Kars and Sarikamiş area by membrane filtration method

In this study, the microbiological quality of drinking water and the presence of Escherichia coli was investigated in the military troops of Kars and Sarıkamış area. In this investigation, faecal indicators (E.coli) were isolated by using membrane filtration methods. This method has been accepted as an alternative method for the detection of fecal coliform organisms comparing with the standard method. For this purpose,1469 water samples were collected and analyzed during 6 months.The samples were filtered through 0.45μm pore size cellulose membrane filter. After filtration, each filter was asceptically placed on the Endo-NKS medium and incubated for 24h at 37 ºC. Growing red colonies on the medium were taken and identified as E.coli by biochemical methods. In conclusion, E.coli was isolated in 439 of the (30%). Because the presence of E.coli in the water samples exhibited existence of fecal contamination in the drinking water of the troops regarding this state would cause infections with the water origin.

PDF

___

Albert MJ, Faruque ASG, Faruque SM, Sack RB, Mahalanabis D, 1999. Case-control study of enteropathogens associated with childhood diarrhoea in Dhaka, Bangladesh. J. Clin. Microbiol. 37 (11), 3458–3464.
Dufour A, Strickland ER, Cabellit VJ, 1981.Membrane Filter Method for Enumerating Escherichia coli. App.Environ.Microbiol.5,1152-1158.
Du X, Chun İ, Shen J, Wu B, Shen Z, 2004.Characterization of florfenicol resistance among calf pathogenic Escherichia coli. FEMMS Microbiology Leters.236:183-189.
Edberg SC, Rice EW, Karlin RJ, Allen MJ, 2000. Escherichia coli: the best biological drinking water indicator for public health protection. Symposium Series, Society for applied microbiology.29,106-116.
Edberg SC, Rice EW, Karlin, RJ, Allen MJ, 2000.Escherichia coli: the best biological drinking water indicator for public health protection. J. Appl. Microbiol. Symp. Suppl. 88 ,106–116.
Evans TM,Waarvick CE, Seidler RJ, Lechevallier MW, 1981.Coliform Species Recovered from Untreated Surface Water and Drinking Water by the Membrane Filter, Standard, and Modified Most-Probable-Number Techniquest. App. Environ. Microbiol.41(3),657-663.
Evans TM,Waarvick CE, Seidler RJ, Lechevallier MW, 1981.Failure of the Most-Probable-Number Technique to Detect Coliforms in Drinking Water and Raw Water Suppliest. App. Environ. Microbiol. 41(1),130-138.
Evans TM, Seidler RJ, Lechevallier MW,1981.Impact of Verification Media and Resuscitation on Accuracy of the Membrane Filter Total Coliform Enumeration Techniquet. App.Environ.Microbiol. 41(5),1141-1151.
Farmer J,1999.Enterobacteriaceae: introduction and identification. İn:Murray PR, Baron EJ, Pfaller MA, (Eds). Manuel of Clinical Microbiology. ASM Pres., 442-459.
Grabow EK, Preez M,1979.Comparison of m-Endo LES, MacConkey, and Teepol Media for Membrane Filtration Counting of Total Coliform Bacteria in Water. App. Environ. Microbiol.38 (3), 351-358.
Green BL, Clausen EM, Litsky W, 1977.Two-Temperature Membrane Filter Method for Enumerating Fecal Coliform Bacteria from Chlorinated Effluents. App.Environ.Microbiol. 33(6),1259-1264.
Licence KR, Oates BA, Reid TMS, 2001.An outbreak of E. coli O157 infection with evidence of spread from animals to man through contamination of a private water supply.Epidemiol. Infect. 126,135–138.
Mcfeters GA, Stuart DG, 1972.Survival of Coliform Bacteria in Natural Waters:Field and Laboratory Studies with Membrane-Filter Chambers. App.Environ.Microbiol. 24(5),805-811.
Nedachin AE, Artemova TZ, Dmitrieva RA, Doskina TV, Talaeva IG, 2005. Problems of epidemic safety of drinking water use by the population of Russia.Gig.Sanit.6, 14-8.
Oğur R, Tekbaş ÖF, 2005.Temel Su Analiz Teknikleri. Aydın Matbacılık. Ankara,32-40.
Payment P, Siemiatycki J, Richardson L, Renaud G, Franco E, Prevost M, 1997.A prospective epidemiological study of gastrointestinal health effects due to the consumption of drinking water. International Journal of Environmental Health Research. 7(1),5-31.
Rose R, Geldreich E, Litsky W, 1975. Improved Membrane Filter Method for Fecal Coliform Analysis. App .Environ. Microbiol. 29(4),532-536.
Said B, Wright F, Nichols GL, Reacher M, Rutter M, (2003). Outbreaks of infectious disease associated with private drinking water supplies in England and Wales,1970-2000.Epidemiol.Infect.130(3), 469-479.
Tobin RS, Lomax P, Kushner DJ, 1980.Comparison of Nine Brands of Membrane Filter and the Most-Probable-Number Methods for Total Coliform Enumeration in Sewage-Contaminated Drinking Water. App. Environ. Microbiol. 40(2),186-191.
World Health Organisation (WHO,2004).Guidelines for Drinking-Water Quality (3thed),Geneva.