Halk Elinde Yetiştirilen Simental, İsviçre Esmeri, Güney Anadolu Kırmızısı ve Boz Irk Sığırlarında Beta- Laktoglobulin Gen Polimorfizminin PCR- RFLP Yöntemi ile Belirlenmesi

Bu çalışmada Türkiyede yetiştirilen sığır ırklarından Simental, İsviçre Esmeri, Güney Anadolu Kırmızısı ve Boz Irk sığırlarında beta- laktoglobulin geninin allel yapılarının restriksiyon fragment uzunluk polimorfizmi (RFLP) yöntemi ile belirlenmesiamaçlanmıştır. Çalışmanınmateryalini, halkelindeyetiştirilen 75 başSimental, 75 başİsviçreEsmeri, 40 baş Güney Anadolu Kırmızısı ve 40 baş Boz Irk sığırı oluşturmuştur. Çalışmada kullanılan DNAlar kandan fenol- kloroform yöntemi ile elde edilmiştir. Beta- laktoglobulin allellerinin belirlenmesinde uygulanan polimeraz zincir reaksiyonu (PCR) işleminitakibeneldeedilenPCRürünleriHaeIII endonükleazenzimiilekesilmiştir.Eldeedilenkesimürünleri%4lükagaroz jel eletroforez yöntemi ile belirlenmiştir. Beta- laktoglubulin geni için en yüksek AA genotip frekansı Simental ırkında, en yüksekBBgenotip frekansıGüneyAnadoluKırmızısı (GAK)ırkındaveenyüksekABgenotip frekansıİsviçreEsmeriırkında görülmüştür. GAK ırkında B allelinin frekansı, Simental ırkında ise A allelinin frekansı en yüksek bulunmuştur. Çalışma sonundaincelenenırklardabeta- laktogrobulinlokusuyönündenHardy- Weinbergdengesinden(HWE)sapmagörülmüştür

Detection of Beta- Lactoglobulin Gene Polymorphism by PCR- RFLP in Simmental, Brown Swiss, South Anatolian Red and Turkish Grey Cattle Breeds Reared Privately

The purpose of this work was to examine the allele structures of beta- lactoglobulin gene with restriction fragmentlength polymorphism (RFLP) method in Simmental, Brown Swiss, South Anatolian Red and Turkish Grey cattle breeds beingraised in Turkey. The material of the study consisted of 75 heads of Simmental, 75 heads of Brown Swiss, 40 heads SouthAnatolian Red and 40 heads Turkish Grey cattle. DNA materials were isolated from blood samples using phenol- chloroformextraction method. In order to determine the beta- lactoglobulin alleles in polymerase chain reaction (PCR) products, thePCR products were digested with HaeIII endonuclease enzyme. The digest products obtained were determined byelectrophoresis using 4% agarose gel. The AA genotypic frequency was found the highest in Simmental breed; the BBgenotypic frequency was found the highest in the South Anatolian Red (SAR) breed and the AB genotypic frequency wasfound the highest in Brown Swiss breed in beta- lactoglobulin gene. In this study, the B allele frequency was found higherthan the A allele in SAR cattle breeds. But, the A allele frequency was found higher than the B allele in the Simmental breed.At the end of this study, a deviation in Hardy- Weinberg equilibrium (HWE) was observed in terms of beta- lactoglobulinlocus in the breeds examined.

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