Cirit Atlarında İnfluenza A Virus Enfeksiyonunun Serolojik ve Moleküler Yöntemlerle Araştırılması

İnfluenza A virus (IAV) insan, at, kanatlı ve birçok memeli türünü enfekte eden, zoonotik karakterli ve oldukça bulaşıcısolunum sistemi patojenidir. Bu çalışmada, geleneksel cirit sporunun yaygın olarak yapıldığı Erzurum ilinde solunum sistemihastalığı belirtileri gösteren atlarda influenza A virusun serolojik ve moleküler yöntemlerle araştırılması amaçlanmıştır. Buamaçla Atatürk Üniversitesi Veteriner Fakültesi Hayvan Hastanesi’ne getirilen 57 at çalışmaya dâhil edilmiştir. Herhangi biraşı geçmişi olmayan atlara ait serum ve burun swap örneklerinde ELISA ve RT-PCR teknikleri kullanılarak influenza A virusenfeksiyonu araştırılmıştır. ELISA testi sonucunda atların %26.3’ü (15/57) seropozitif olarak belirlenmiştir. Erkek atların%29.8’inde (14/47) İnfluenza A virusa spesifik antikor varlığı belirlenirken, dişi atların %10’unda (1/10) pozitiflik tespitedilmiştir. Moleküler analizler sonucunda IAV'un RNA'sı bu çalışmada tespit edilmemiştir. Bu çalışma ile bölgemiz cirit atıpopulasyonunda influenza A virus enfeksiyonu araştırılmış ve solunum sistemi problemli atlarda virusa karşı oluşmuşantikorların varlığı serolojik olarak belirlenmiştir. Sonuç olarak virusun bölgemizdeki cirit atlarında indirekt (antikor) olaraksirküle olduğu ortaya konulmuştur. Bu sporla uğraşan ve at yetiştiriciliği yapan işletmelerde enfeksiyonun var olabileceğibelirlenmiştir. Cirit atlarında influenza virusun varlığının belirlendiği, belirlenen virusun tiplendirildiği ve bu hastalıktankorunma tedbirlerine yönelik daha kapsamlı çalışmaların yapılması önerilmektedir.

Investigation of Influenza A Virus Infection by Serological and Molecular Methods in Jereed Horses

Influenza A virus (IAV) is a zoonotic and highly contagious pathogen of respiratory system infecting humans, horses, poultry and many mammals. In this study, it was aimed to investigate the influenza A virus by serological and molecular methods in horses with signs of respiratory system in province of Erzurum, where traditional jereed sport is widely held. For this purpose, 57 horses were admitted to Atatürk University Veterinary Faculty Animal Hospital were included in this study. IAV infection was investigated by using ELISA and RT-PCR techniques in serum and nasal swab samples of horses without any vaccine. As a result of ELISA test, 26.3% of the horses (15/57) were determined to be seropositive.Antibodies against IAV were detected in 14 (29.8%) of the male horses, whereas only 1 (10%) female horse had antibody. As a result of molecular analysis, the RNA of IAV was not detected in this study. In this study, we investigated the influenza A virus infection in the jereed horse population in our region and the presence of antibodies against virus in horses with respiratory system was determined serologically. As a result, it has been shown that the virus is circulated indirectly (antibody) in jereed horses in our region. The presence of infection in horse-breeding farms engaged in this sport was determined. It is recommended to perform more comprehensive studies on diagnosis and type identification of influenza virus and determination of the protection measures in jereed horses.

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