Nurten DİKMEN, Hülya LEVENTERLER, İbrahim Ferhat ÜRÜNSAK

Dipeptidil Peptidaz- 4 Enzimine Biyokimyasal Yaklaşım

Dipeptidil peptidaz- 4 (DPP-4, EC 3.4. 14.5 ), proteaz ailesinin bir üyesidir. DPP-4 aynı zamanda adenozin deaminazbağlayan protein (ADBP) ya da T-hücre aktivasyon antijeni CD26 olarak da bilinmektedir. Bir serin ekzopeptidaz olanDPP-4; bir proteinin N-terminal dipeptitin en sonunda yer alan prolin, hidroksiprolin, dehidroprolin ya da alaninayrılmasını katalizlemektedir. Enzim kanda, serbest ve bağlı olmak üzere iki formda bulunmaktadır.DPP-4’ün pek çok dokudaki yaygın dağılımı ve fizyolojik olaylarda rol almasına bağlı olarak çeşitli patolojik durumlardada DPP-4 ekspresyonunda ya da aktivitesinde değişiklikler saptanmıştır. Bu patolojik durumlar: diyabet, psikonöroendokrin bozukluklar, otoimmün ve inflamatuar hastalıklar, enfeksiyon hastalıkları, hematolojik malignansiler,solid tümörlerdir. Plazmada değişen CD26/DPP-4 aktivitesi ya da derişimleri fizyolojik ve otoimmün çeşitli patolojikdurumlarla ilgili bulunmuştur. Ayrıca tükürük, serebrospinal, sinoviyal sıvılar, folikül sıvısı, semen ve idrarda da DPP4 aktivitesi bildirilmiştir.

Biochemical Approach to Dipeptidyl Peptidase-4 Enzyme

Dipeptidyl peptidase-4 (DPP-4, EC 3.4. 14.5 ) is a member of protease family. DPP-4, also known as adenosine deaminase binding protein (ADBP) or T-cell activation antigen CD26. DPP-4 is a serine exopeptidase, it cleaves dipeptides from the N-termini of oligopeptides and smaller peptides with proline, hydroxyproline, dehydroproline or alanine. The enzyme is found in two forms in blood, free and bound. Due to its ubiquitous distribution and involvement in various physiological processes, a great number of pathological conditions are associated with altered DPP-4 expression or activity. These pathological conditions are: diabetes, psychoneuroendocrine disorders, autoimmune and inflammatory diseases, infectious diseases, haematological malignancies, solid tumors. The altered CD26 / DPP-4 activity or concentrations in plasma are related to various physiological and autoimmune pathological conditions. DPP-4 activity is detected in saliva, cerebrospinal fluid, synovial fluid, follicular fluid, seminal fluid and urine.

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