Muammer GÖNCÜOĞLU, Alireza HANIFEHNEZHAD, İrfan EROL

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Bu çalışmada yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve elde edilen izolatların molekülerkarakterizasyonlarının belirlenmesi amaçlanmıştır. Çalışmada farklı süpermarketlerde satışa sunulan 45 kalp-karaciğer ve 45 taşlıkolmak üzere toplam 90 yenilebilir iç organ soğuk zincir altında laboratuvara getirilerek aynı gün içerisinde analize alınmıştır.Örneklerden C. perfringens izolasyon ve identifikasyonunda klasik kültür tekniği ile asit fosfataz ve reverse -CAMP gibibiyokimyasal testler kullanılmıştır. Kırk beş kalp-karaciğer örneğinin 21inin (% 46.7) ve 45 taşlık örneğinin sekizinin (% 17.7) C. perfringens ile kontamine olduğu saptanmıştır. Elde edilen izolatların alfa (cpa ), beta (cpb ), beta 2 (cpb2 ), epsilon (etx), iota (iA) veenterotoksin (cpe ) genlerine ait primer dizilimleri kullanılarak multipleks PCR ile moleküler tiplendirilmeleri yapılmıştır. Sonuçlarincelendiğinde bütün izolatların cpa geni taşıdığı belirlenmiş ve tip A olarak sınıflandırılmıştır, bununla beraber hiçbir izolatın cpe genine sahip olmadığı saptanmıştır. İki farklı taşlık örneğinden elde edilen izolatların cpa yanında cpb2 genini de taşıdıklarıbelirlenmiştir. Sonuç olarak yenilebilir piliç iç organlarının dünya genelinde gıda zehirlenmelerinde en sıklıkla rastlanan C.perfringens tip A ile yüksek oranda kontamine olduğu belirlenmiştir. Yüksek kontaminasyona bağlı olarak yenilebilir piliç içorganlarının C. perfringens tip A zehirlenmeleri açısından önemli birer kaynak oldukları saptanmıştır.

Yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve moleküler tiplendirilmesi

The aim of the study was to determine the prevalance and molecular characterization of Clostridium perfringens in edible offal of broiler. For this purpose a total of 90 samples, including 45 heart-livers and 45 gizzards were obtained fromdifferent supermarkets and transported to the laboratory in an ice box and tested at the same day. Culture technique was used for theisolation and selected biochemical tests, including acid phosphatase and reverse-CAMP tests was used for the identification of C.perfringens from the samples. Twenty one (46.7%) of 45 heart-liver and eight (17.7%) of 45 gizzards were found to be c ontaminatedwith C. perfringens. Isolates were molecular characterized by using multiplex PCR with alpha (cpa ), beta (cpb ), beta 2 (cpb2 ),epsilon (etx), iota (iA) and enterotoxin (cpe ) genes. Results showed that all isolates were carrying cpa gene and nam ed as type A butnone of them were positive for cpe gene. Only two gizzard isolates were positive for cpb2 gene. Our results indicate that edible offalof broiler are highly contaminated with C. perfringens type A which is mostly responsible for the foodbo rne poisoning worldwide.According to the high contamination rate edible offal should be considered as one of the important causes of C. perfringens type Afoodborne disease.

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