Pınar Alkım ULUTAŞ, Funda KIRAL, Mürüvvet URAL

Procalcitonine, Nitric oxide and C - Reactive Protein concentrations in rats with experimentally- induced infectious and noninfectious inflammation

Bu çalışmanın amacı, infeksiyöz ve noninfeksiyöz yangı durumunda prokalsitonin(PCT), Nitrik oksit (NO) ve C -reaktifprotein (CRP) düzeylerini belirlemek ve yangının ayrımının yapılmasında kullanılabilirliğini değerlendirmektir. Bu çalışmada toplamolarak 250 -300 gram ağırlığında 70 dişi Wistar rat (Grup 1: n=21, Grup 2: n=21, Grup 3: n=21 ve 0. Gün n=7) kullanıldı.Uygulamalardan önce 7 rattan kan örnekleri alınıp 0. gün olarak kabul edildi. Ayrıca her biri 21 rat içeren 3 grup oluşturuldu.İnfeksiyon grubunda (Grup 1) bulunan 21 rata Staphylococcus aureus 10 6 CFU derialtı olarak verildi. Noninfeksiyöz yangı grubundabulunan 21 rata Turbentin yağı 0 .5 mg/kg derialtı uygulandı. Kontrol grubundaki 21 rata derialtı fizyolojik tuzlu su verildi.Uygulamalardan sonra her gruptan rastgele seçilen yedişer hayvandan, 1., 4. ve 7. günlerde kan örnekleri toplandı. PCT, CRP v e NOdüzeyleri ELISA cihazında ticari test kitleriyle çalışıldı. Elde edilen sonuçlara göre PCT, NO ve CRP düzeylerinin uygulamalardansonra arttığını belirlendi. CRP ve PCT düzeylerindeki artış infeksiyöz grupta diğer iki gruba göre daha fazlaydı ancak istatistiki önemsaptanamadı. Bu çalışmanın sonuçlarına dayanarak, NOin infeksiyöz yangıda önemli düzeyde arttığı ve kliniklerde ayırıcı tanıamaçlı kullanılabileceği söylenebilir.

Deneysel olarak infeksiyöz ve noninfeksiyöz yangı oluşturulmuş ratlarda prokalsitonin, nitrik oksit ve C- reaktif protein konsantrasyonları

The aim of this study is to determine the alterations in procalcitonine (PCT), nitric oxide (NO) and C-reactiveprotein (CRP) levels in infectious and noninfectious inflammation and to evaluate their potential in the differentiation ofinflammation. Totally 70 female Wistar rats (Group 1: n=21; Group 2, n=21, Group 3: n=21; Day 0: n=7), weighing 250 -300 grams,were used in this study. Before the applications, blood samples were collected in seven rats and the time is accepted as Day 0. Threegroups were formed each containing 21 rats. Stapylococcus aureus were administered at a concentration of 10 6 CFU in the infectiongroup (Group 1), subcutaneo usly. Turbentine oil at a dose of 0.5 mg/kg were injected subcutaneously to twenty -one rats in thenoninfectious inflammation group (Group 2). In the control group, 21 rats were injected with saline subcutaneously. Blood sam pleswere collected from randomly selected 7 rats in each group on the 1 st, 4 th and the 7 th days . PCT, NO, and CRP analyzes wereperformed with commercial test kits in the ELISA reader. According to the obtained results, we determined that serum PCT, NO andCRP levels increased after administrations. The rises of CRP and PCT concentrations were not statistically significant but it washigher in infectious group levels than the other two groups. Based on these results, it can be said that NO shows significant increasesduring infectious inflammation and can be used for diferential diagnostic purposes in clinics.

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Geliş tarihi: 10.03.2014 / Kabul tarihi: 14.07.2014