Mecit YÖRÜK, Sema USLU

3845

A morphological and histometric study on the distribution and heterogeneity of mast cells found in lungs and trachea of Van Cats

Bu çalışma Van Kedilerinde trakeya ve akciğerlerde bulunan mast hücrelerinin dağılımı ve heterojenitesini belirlemekamacıyla yapıldı. Çalışmada 4 adet Van Kedisi kullanıldı. Van Kedilerinin trakeya ve akciğerlerinden uygun büyüklükte parçala ralındı. BLA (Basic Kurşun Asetat-Mota)da tespit edildikten sonra rutin doku takibi yapılarak paraplast ile bloklandı. 6 -7 µmkalınlığında alınan kesitler % 0,5lik toluidine blue ve alcian blue -safranin O kombine boyalarında boyandılar. Toluidine blue ileboyanan kesitlerde 40lık objektifte mast hücre sayıları semikantitatif olarak belirlendi. İncelenen organlardan akciğerde en fazlasayıda mast hücresine rastlandı. Granül yapısını belirlemede BLA tespitinin uygun olduğu saptandı. Alcian blue - safranin Oboyamasında ise trakeya ve akciğerde SO (+), AB (+) granüllü mast hücrelerine rastlandı, miks granüle sahip mast hücre lerine iserastlanılamadı.

Van Kedilerinin trakeya ve akciğerlerinde bulunan mast hücrelerinin dağılımı ve heterojenitesi üzerine morfolojik ve histometrik çalışma

This study was performed to specify the distribution and the heterogeneity of mast cells found in lungs andtrachea of Van Cats. Four Van Cats were used in the study. Appropriate siz ed pieces were taken from the lungs and tracheas of thecats. After BLA (Basic Lead Acetate-Mota) was set, it was blocked with paraplast by doing the routine tissue follow -up. Sectionstaken in 6 -7 μm thickness were stained with 0,5 % toluidine blue and alcian -blue safranin O combined stain. Numbers of mast cellswere specified semi-quantitatively in the sections stained with toluidine blue under lens 40. Among analysed organs, the most mastcells were found in lungs. To specify the granule construction, BLA detection was determined to be suitable. In the stain of Alcian - blue safranin O, SO (+), AB (+) granulose mast cells were found; mast cells that have mixed granules were not found.
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Ankara Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • Yayın Aralığı: 4
  • Başlangıç: 1954
  • Yayıncı: Ankara Üniversitesi Veteriner Fakültesi
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