Nergis CANTÜRK, Miyase ODABAŞI, Zeliha KAYAALTI, Levent ŞAHİNER, Tülin SÖYLEMEZOĞLU

Detection of mitochondrial DNA deletions in heart tissue with acute myocardial infarction

Amaç: Bu çalışmanın amacı, 4977 bp ve 7436 bp mitokondriyal DNA (mtDNA) delesyonlarını belirlemek ve otopsi kalp kası örneklerindeki miyokardiyal infarktüs (MI) ve mtDNA delesyonları arasında ilişki olup olmadığını araştırmaktır. Materyal ve Metod: Çalışmada 33 MI’lı ve 33 MI’sız kişiden alınan toplam 66 otopsi kalp dokusu kullanıldı. 4977 ve 7436 bp mtDNA delesyonlarını belirlemek için, kontrollerin kalp kaslarından ve MI’lı kalp dokularından mtDNA izole edildi ve polimeraz zincir reaksiyonu (PCR) tekniği ile çoğaltıldı. Çoğaltılan ürünler %2’lik agaroz jel elektroforezinde ayrıştırıldı. Bulgular: MI’lı kişilerin kalp doklarının %97’sinde 4977 bp mtDNA delesyonu gözlemlendi. Diğer taraftan kontrol grubunda bu oran sadece %39.4’dü (p

Akut miyokardiyal infarktüslü kalp dokularında mitokondriyal DNA delesyonlarının belirlenmesi

Aim: The aim of this study was to determine 4977 bp and 7436 bp mitochondrial DNA (mtDNA) deletions and to investigate whether there is an association between the mtDNA deletions and myocardial infarction (MI) in cardiac muscle samples from autopsies. Material and Method: In this study, a total number of 66 heart tissues from autopsies were studied, 33 of them were obtained from individuals with MI and the rest without MI. In order to determine 4977 and 7436 bp mtDNA deletions, mtDNAs in heart muscles from controls and heart tissues from MI region were isolated and amplified with polymerase chain reaction (PCR) technique. The amplified products separated on 2% agarose gel electrophoresis. Results: 4977 bp mtDNA deletion was observed in 97% of the heart tissues of the individuals with MI. On the other hand, this ratio was only 39.4% for the control group (p

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