Hayati YÜKSEL, Yaşar ENLİ, Günfer TURGUT, Emin Oğuzhan OĞUZ, Havane ENLİ, Onur ZORBOZAN, Zuhal CAN

Aluminyum Sülfat’ın "Ross" cinsi term besi civcivi karaciğerinde yarattığı toksik ve inflamatuar hasar

Amaç: Döllenmiş Ross cinsi besi tavuk yumurtalarına kuluçkanın 1.gününde toksik dozda verilen Aluminyum Sülfat ın term civciv karaciğerinde yaratmış olduğu muhtemel histolojik ve biyokimyasal etkilerin gösterilmesi Gereç ve Yöntem: Döllenmiş Ross cinsi besi yumurtaları üç gruba ayrılarak deney grubu hava boşluğuna kuluçkanın 1.gününde 1000 mcg/0.1 ml serum fizyolojik içinde Aluminyum sülfat verildi. Sham grubuna sadece 0.1 ml serum fizyolojik uygulanırken, Kontrol grubuna herhangi bir işlem yapılmadı. Terme ulaşan civcivlerden karaciğerler alınarak ışık mikroskobu ile histolojik değişikliklere ve biyokimyasal olarak oksidatif reaksiyon indikatörleri olan Malondaialdehit (MDA) ve Glutatyon (GSH) düzeylerine bakıldı. Bulgular: Işık mikroskobunda deney grubunda yaygın inflamasyona, hepatosit hasarına, santral ven duvarında kalınlaşma ve pasif konjesyona ve sinuzoid yapısında bozulmaya rastlandı. Biyokimyasal olarak ise deney grubunda oksidatif reaksiyonu gösteren MDA yüksekliği ve GSH tükenmesi verilen dozda term Ross besi civcivlerinde ilk kez gösterildi. Hem histolojik hem de biyokimyasal olarak Sham ve Kontrol gruplarında herhangi bir değişikliğe rastlanmadı. Sonuç: Döllenmiş Ross cinsi besi tavuk yumurtalarına kuluçkanın 1.gününde 1000 mcg/0.1ml serum fizyolojik içinde verilen Aluminyum Sülfat term civciv karaciğerinde yaygın inflamatuar ve oksidatif hasara yol açmıştır.

Oxidative and inflamatory of alüminium suplhate on the liver of term Ross broiler chick

Aim: To show histological and biochemical effects of toxic dose Aluminium Sulphate injected on the day 1 in the liver of term Ross broiler chicks. Materials and Methods: Fertilised Ross broiler chicken eggs which reached term were divided into three groups and experimental group were injected by 1000 mcg/0.1 ml saline into their air chambers. Sham group eggs were injected by saline only and control group eggs were not applied anything. After incubation, at term, on the day 21, surviving chicks were sacrificed, livers taken and histological and biochemical investigation were performed. A lobe of the livers were Hema-toxylen Eosin stained and observed under light microscopy. Other lobe of the livers were used to determine Malondialdehyde (MDA) and Gluthation (GSH) levels. Findings: First time in term Ross broiler chicks, in experimental group, massive in liver inflamatory changes, sinusoidal impairment and hepatocyte specific pathologies were seen under light microscopy examination. As an oxidative damage indicators tremendous MDA level increase and GSH level decrease were observed. In sham and control groups normal liver histology and normal MDA and GSH levels were observed. Conclusion: In experimental group, toxic dose Aluminium Sulphate injected into the air chambers of Ross broiler chicken eggs on the day 1 showed massive histological inflamatory damage and increased MDA and decreased GSH levels at term chick livers.

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